G9a and Histone Deacetylases Are Crucial for Snail2-mediated E-cadherin Repression and Metastasis in Hepatocellular Carcinoma

Overview

Journal Cancer Sci

Specialty Oncology

Date 2019 Aug 22

PMID 31432592

Citations 30

Authors

Yue Hu

Yayuan Zheng

Mingrui Dai

Xueju Wang

Jiaxin Wu

Bin Yu

Haihong Zhang

Yinqiu Cui

Wei Kong

Hui Wu

Xianghui Yu

Affiliations

Soon will be listed here.

Abstract

Functional E-cadherin loss, a hallmark of epithelial-mesenchymal transition (EMT), is important for metastasis. However, the mechanism of Snail2 in hepatocellular carcinoma (HCC) EMT and metastasis remains unclear. Here, we showed that Snail2 was upregulated in primary HCC, and significantly increased during transforming growth factor-β-induced liver cell EMT. Snail2-overexpressing and knockdown cell lines have been established to determine its function in EMT in HCC. H3K9 methylation was upregulated and H3K4 and H3K56 acetylation were downregulated at the E-cadherin promoter in Snail2-overexpressing cancer cells. Furthermore, Snail2 interacted with G9a and histone deacetylases (HDACs) to form a complex to suppress E-cadherin transcription. Snail2 overexpression enhanced migration and invasion in HCC cells, whereas G9a and HDAC inhibition significantly reversed this effect. Moreover, Snail2 overexpression in cancer cells increased tumor metastasis and shortened survival time in mice, whereas G9a and HDAC inhibitors extended survival. Our study not only reveals a critical mechanism underlying the epigenetic regulation of EMT but also suggests novel treatment strategies for HCC.

Citing Articles

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