Human CD4CD103 Cutaneous Resident Memory T Cells Are Found in the Circulation of Healthy Individuals

Overview

Journal Sci Immunol

Specialty Allergy & Immunology

Date 2019 Jul 7

PMID 31278120

Citations 120

Authors

Maria M Klicznik

Peter A Morawski

Barbara Hollbacher

Suraj R Varkhande

Samantha J Motley

Leticia Kuri-Cervantes

Eileen Goodwin

Michael D Rosenblum

S Alice Long

Gabriele Brachtl

Thomas Duhen

Michael R Betts

Daniel J Campbell

Iris K Gratz

Affiliations

Soon will be listed here.

Abstract

Tissue-resident memory T cells (T) persist locally in nonlymphoid tissues where they provide frontline defense against recurring insults. T at barrier surfaces express the markers CD103 and/or CD69, which function to retain them in epithelial tissues. In humans, neither the long-term migratory behavior of T nor their ability to reenter the circulation and potentially migrate to distant tissue sites has been investigated. Using tissue explant cultures, we found that CD4CD69CD103 T in human skin can down-regulate CD69 and exit the tissue. In addition, we identified a skin-tropic CD4CD69CD103 population in human lymph and blood that is transcriptionally, functionally, and clonally related to the CD4CD69CD103 T population in the skin. Using a skin xenograft model, we confirmed that a fraction of the human cutaneous CD4CD103 T population can reenter circulation and migrate to secondary human skin sites where they reassume a T phenotype. Thus, our data challenge current concepts regarding the strict tissue compartmentalization of CD4 T cell memory in humans.

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