The Impact of TCR Signal Strength on Resident Memory T Cell Formation During Influenza Virus Infection

Overview

Journal J Immunol

Specialty Allergy & Immunology

Date 2019 Jun 26

PMID 31235552

Citations 26

Authors

Jessica K Fiege

Ian A Stone

Elizabeth J Fay

Matthew W Markman

Sathi Wijeyesinghe

Marissa G Macchietto

Steven Shen

David Masopust

Ryan A Langlois

Affiliations

Soon will be listed here.

Abstract

Resident memory T cells (T) in the lung are vital for heterologous protection against influenza A virus (IAV). Environmental factors are necessary to establish lung T; however, the role of T cell-intrinsic factors like TCR signal strength have not been elucidated. In this study, we investigated the impact of TCR signal strength on the generation and maintenance of lung T after IAV infection. We inserted high- and low-affinity OT-I epitopes into IAV and infected mice after transfer of OT-I T cells. We uncovered a bias in T formation in the lung elicited by lower affinity TCR stimulation. TCR affinity did not impact the overall phenotype or long-term maintenance of lung T Overall, these findings demonstrate that T formation is negatively correlated with increased TCR signal strength. Lower affinity cells may have an advantage in forming T to ensure diversity in the Ag-specific repertoire in tissues.

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