Induced Pluripotent Stem Cell-Derived Brain Endothelial Cells As a Cellular Model to Study Infection

Meningococcal meningitis is a severe central nervous system infection that occurs when () penetrates brain endothelial cells (BECs) of the meningeal blood-cerebrospinal fluid barrier. As a human-specific pathogen, models are greatly limited and pose a significant challenge. cell models have been developed, however, most lack critical BEC phenotypes limiting their usefulness. Human BECs generated from induced pluripotent stem cells (iPSCs) retain BEC properties and offer the prospect of modeling the human-specific interaction with BECs. Here, we exploit iPSC-BECs as a novel cellular model to study host-pathogen interactions, and provide an overview of host responses to infection. Using iPSC-BECs, we first confirmed that multiple strains and mutants follow similar phenotypes to previously described models. The recruitment of the recently published pilus adhesin receptor CD147 underneath meningococcal microcolonies could be verified in iPSC-BECs. was also observed to significantly increase the expression of pro-inflammatory and neutrophil-specific chemokines , , , , and , and the secretion of IFN-γ and RANTES. For the first time, we directly observe that disrupts the three tight junction proteins ZO-1, Occludin, and Claudin-5, which become frayed and/or discontinuous in BECs upon challenge. In accordance with tight junction loss, a sharp loss in endothelial electrical resistance, and an increase in sodium fluorescein permeability and in bacterial transmigration, was observed. Finally, we established RNA-Seq of sorted, infected iPSC-BECs, providing expression data of -responsive host genes. Altogether, this model provides novel insights into pathogenesis, including an impact of on barrier properties and tight junction complexes, and suggests that the paracellular route may contribute to traversal of BECs.