Group B Transcriptome when Interacting with Brain Endothelial Cells

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2024 May 21

PMID 38771039

Authors

Nadine Vollmuth

Bailey E Bridgers

Madelyn L Armstrong

Jacob F Wood

Abigail R Gildea

Eric R Espinal

Thomas A Hooven

Giulia Barbieri

Alexander J Westermann

Till Sauerwein

Konrad U Foerstner

Alexandra Schubert-Unkmeir

Brandon J Kim

Affiliations

Soon will be listed here.

Abstract

Importance: Group B (GBS) meningitis remains the leading cause of neonatal meningitis. Research work has identified surface factors and two-component systems that contribute to GBS disruption of the blood-brain barrier (BBB). These discoveries often relied on genetic screening approaches. Here, we provide transcriptomic data describing how GBS changes its transcriptome when interacting with brain endothelial cells. Additionally, we have phenotypically validated these data by obtaining mutants of a select regulator that is highly down-regulated during infection and testing on our BBB model. This work provides the research field with a validated data set that can provide an insight into potential pathways that GBS requires to interact with the BBB and open the door to new discoveries.

Citing Articles

Common Chemical Plasticizer Di(2-Ethhylhexyl) Phthalate Exposure Exacerbates Coxsackievirus B3 Infection.

Kordbacheh R, Ashley M, Cutts W, Keyzer T, Chatterjee S, Altman T Viruses. 2025; 16(12.

PMID: 39772131 PMC: 11680387. DOI: 10.3390/v16121821.

Transcriptomic analysis of coxsackievirus B3 infection in induced pluripotent stem cell-derived brain-like endothelial cells.

Hathcock S, Mamana J, Keyzer T, Vollmuth N, Shokri M, Mauser H J Virol. 2024; 99(1):e0182424.

PMID: 39670741 PMC: 11784093. DOI: 10.1128/jvi.01824-24.

strain COH1 transcriptome in association with stem cell-derived brain-like endothelial cells.

Vollmuth N, Sauerwein T, Foerstner K, Westermann A, Schubert-Unkmeir A, Lam D Microbiol Resour Announc. 2024; 13(12):e0045524.

PMID: 39526785 PMC: 11636360. DOI: 10.1128/mra.00455-24.

References

Lippmann E, Al-Ahmad A, Azarin S, Palecek S, Shusta E . A retinoic acid-enhanced, multicellular human blood-brain barrier model derived from stem cell sources. Sci Rep. 2014; 4:4160. PMC: 3932448. DOI: 10.1038/srep04160. View

Love M, Huber W, Anders S . Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol. 2014; 15(12):550. PMC: 4302049. DOI: 10.1186/s13059-014-0550-8. View

Doran K, Banerjee A, Disson O, Lecuit M . Concepts and mechanisms: crossing host barriers. Cold Spring Harb Perspect Med. 2013; 3(7). PMC: 3685877. DOI: 10.1101/cshperspect.a010090. View

Kim K . Microbial translocation of the blood-brain barrier. Int J Parasitol. 2006; 36(5):607-14. DOI: 10.1016/j.ijpara.2006.01.013. View

Zhu L, Beres S, Yerramilli P, Pruitt L, Cantu C, Olsen R . Genetic Basis Underlying the Hyperhemolytic Phenotype of Streptococcus agalactiae Strain CNCTC10/84. J Bacteriol. 2020; 202(23). PMC: 7648153. DOI: 10.1128/JB.00504-20. View

Nizet V, Kim K, Stins M, Jonas M, Chi E, Nguyen D . Invasion of brain microvascular endothelial cells by group B streptococci. Infect Immun. 1997; 65(12):5074-81. PMC: 175731. DOI: 10.1128/iai.65.12.5074-5081.1997. View

Edwards M, Baker C . Group B Streptococcal Disease: Interim Prevention at 50 Years and Counting. Clin Infect Dis. 2019; 70(12):2580-2581. DOI: 10.1093/cid/ciz738. View

Rahman N, Rasil A, Meyding-Lamade U, Craemer E, Diah S, Tuah A . Immortalized endothelial cell lines for in vitro blood-brain barrier models: A systematic review. Brain Res. 2016; 1642:532-545. DOI: 10.1016/j.brainres.2016.04.024. View

Stenz L, Francois P, Whiteson K, Wolz C, Linder P, Schrenzel J . The CodY pleiotropic repressor controls virulence in gram-positive pathogens. FEMS Immunol Med Microbiol. 2011; 62(2):123-39. DOI: 10.1111/j.1574-695X.2011.00812.x. View

10.

Kim B, McDonagh M, Deng L, Gastfriend B, Schubert-Unkmeir A, Doran K . Streptococcus agalactiae disrupts P-glycoprotein function in brain endothelial cells. Fluids Barriers CNS. 2019; 16(1):26. PMC: 6704684. DOI: 10.1186/s12987-019-0146-5. View

11.

Seo H, Mu R, Kim B, Doran K, Sullam P . Binding of glycoprotein Srr1 of Streptococcus agalactiae to fibrinogen promotes attachment to brain endothelium and the development of meningitis. PLoS Pathog. 2012; 8(10):e1002947. PMC: 3464228. DOI: 10.1371/journal.ppat.1002947. View

12.

Pellegrini A, Lentini G, Fama A, Bonacorsi A, Scoffone V, Buroni S . CodY Is a Global Transcriptional Regulator Required for Virulence in Group B . Front Microbiol. 2022; 13:881549. PMC: 9096947. DOI: 10.3389/fmicb.2022.881549. View

13.

Le Doare K, Heath P . An overview of global GBS epidemiology. Vaccine. 2013; 31 Suppl 4:D7-12. DOI: 10.1016/j.vaccine.2013.01.009. View

14.

Rubens C, Wessels M, Heggen L, Kasper D . Transposon mutagenesis of type III group B Streptococcus: correlation of capsule expression with virulence. Proc Natl Acad Sci U S A. 1987; 84(20):7208-12. PMC: 299259. DOI: 10.1073/pnas.84.20.7208. View

15.

Feng L, Zhu J, Chang H, Gao X, Gao C, Wei X . The CodY regulator is essential for virulence in Streptococcus suis serotype 2. Sci Rep. 2016; 6:21241. PMC: 4756307. DOI: 10.1038/srep21241. View

16.

Endres L, Hathcock S, Schubert-Unkmeir A, Kim B . Neisseria meningitidis Infection of Induced Pluripotent Stem-Cell Derived Brain Endothelial Cells. J Vis Exp. 2020; (161). DOI: 10.3791/61400. View

17.

Minhas V, Harvey R, McAllister L, Seemann T, Syme A, Baines S . Capacity To Utilize Raffinose Dictates Pneumococcal Disease Phenotype. mBio. 2019; 10(1). PMC: 6336424. DOI: 10.1128/mBio.02596-18. View

18.

Banerjee A, Kim B, Carmona E, Cutting A, Gurney M, Carlos C . Bacterial Pili exploit integrin machinery to promote immune activation and efficient blood-brain barrier penetration. Nat Commun. 2011; 2:462. PMC: 3195231. DOI: 10.1038/ncomms1474. View

19.

Otto C, Stadler P, Hoffmann S . Lacking alignments? The next-generation sequencing mapper segemehl revisited. Bioinformatics. 2014; 30(13):1837-43. DOI: 10.1093/bioinformatics/btu146. View

20.

Doran K, Fulde M, Gratz N, Kim B, Nau R, Prasadarao N . Host-pathogen interactions in bacterial meningitis. Acta Neuropathol. 2016; 131(2):185-209. PMC: 4713723. DOI: 10.1007/s00401-015-1531-z. View