A Repetitive Antigen of Plasmodium Falciparum That is Homologous to Heat Shock Protein 70 of Drosophila Melanogaster

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1986 Nov 1

PMID 3095842

Citations 43

Authors

A E Bianco

J M Favaloro

T R Burkot

J G Culvenor

P E Crewther

G V Brown

R F Anders

R L Coppel

D J Kemp

Affiliations

Soon will be listed here.

Abstract

We describe an antigen of Plasmodium falciparum, defined by a cDNA clone designated Ag63. The antigen is an abundant, soluble cytoplasmic polypeptide of Mr 75,000 present in all stages of asexual development in the blood and in gametocytes, but not in sporozoites. The sequence of the cDNA clone revealed that, like many other antigens of P. falciparum, it contains tandemly repeated amino acid sequences, in this case Gly-Gly-Met-Pro. However, the rest of the sequence is 70% homologous at the amino acid level to the heat shock protein hsp70 of Drosophila melanogaster.

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References

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

Ingolia T, Craig E, McCarthy B . Sequence of three copies of the gene for the major Drosophila heat shock induced protein and their flanking regions. Cell. 1980; 21(3):669-79. DOI: 10.1016/0092-8674(80)90430-4. View

Ingolia T, Craig E . Drosophila gene related to the major heat shock-induced gene is transcribed at normal temperatures and not induced by heat shock. Proc Natl Acad Sci U S A. 1982; 79(2):525-9. PMC: 345776. DOI: 10.1073/pnas.79.2.525. View

Messing J, Vieira J . A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene. 1982; 19(3):269-76. DOI: 10.1016/0378-1119(82)90016-6. View

Ingolia T, Slater M, Craig E . Saccharomyces cerevisiae contains a complex multigene family related to the major heat shock-inducible gene of Drosophila. Mol Cell Biol. 1982; 2(11):1388-98. PMC: 369943. DOI: 10.1128/mcb.2.11.1388-1398.1982. View

Howard R, Barnwell J, Kao V, Daniel W, Aley S . Radioiodination of new protein antigens on the surface of Plasmodium knowlesi schizont-infected erythrocytes. Mol Biochem Parasitol. 1982; 6(6):343-67. DOI: 10.1016/0166-6851(82)90024-x. View

Dayhoff M, Barker W, Hunt L . Establishing homologies in protein sequences. Methods Enzymol. 1983; 91:524-45. DOI: 10.1016/s0076-6879(83)91049-2. View

Kemp D, Coppel R, Cowman A, Saint R, Brown G, Anders R . Expression of Plasmodium falciparum blood-stage antigens in Escherichia coli: detection with antibodies from immune humans. Proc Natl Acad Sci U S A. 1983; 80(12):3787-91. PMC: 394137. DOI: 10.1073/pnas.80.12.3787. View

GODSON G, Ellis J, Svec P, Schlesinger D, NUSSENZWEIG V . Identification and chemical synthesis of a tandemly repeated immunogenic region of Plasmodium knowlesi circumsporozoite protein. Nature. 1983; 305(5929):29-33. DOI: 10.1038/305029a0. View

10.

Coppel R, Cowman A, Lingelbach K, Brown G, Saint R, Kemp D . Isolate-specific S-antigen of Plasmodium falciparum contains a repeated sequence of eleven amino acids. Nature. 1983; 306(5945):751-6. DOI: 10.1038/306751a0. View

11.

HEIDRICH H, Strych W, Mrema J . Identification of surface and internal antigens from spontaneously released Plasmodium falciparum merozoites by radio-iodination and metabolic labelling. Z Parasitenkd. 1983; 69(6):715-25. DOI: 10.1007/BF00927421. View

12.

Bardwell J, Craig E . Major heat shock gene of Drosophila and the Escherichia coli heat-inducible dnaK gene are homologous. Proc Natl Acad Sci U S A. 1984; 81(3):848-52. PMC: 344935. DOI: 10.1073/pnas.81.3.848. View

13.

Dame J, Williams J, McCutchan T, Weber J, Wirtz R, Hockmeyer W . Structure of the gene encoding the immunodominant surface antigen on the sporozoite of the human malaria parasite Plasmodium falciparum. Science. 1984; 225(4662):593-9. DOI: 10.1126/science.6204383. View

14.

Coppel R, Cowman A, Anders R, Bianco A, Saint R, Lingelbach K . Immune sera recognize on erythrocytes Plasmodium falciparum antigen composed of repeated amino acid sequences. Nature. 1984; 310(5980):789-92. DOI: 10.1038/310789a0. View

15.

Koenen M, Scherf A, Mercereau O, Langsley G, Sibilli L, Dubois P . Human antisera detect a Plasmodium falciparum genomic clone encoding a nonapeptide repeat. Nature. 1984; 311(5984):382-5. DOI: 10.1038/311382a0. View

16.

Hunter K, Cook C, Hayunga E . Leishmanial differentiation in vitro: induction of heat shock proteins. Biochem Biophys Res Commun. 1984; 125(2):755-60. DOI: 10.1016/0006-291x(84)90603-x. View

17.

Stahl H, Crewther P, Anders R, Brown G, Coppel R, Bianco A . Interspersed blocks of repetitive and charged amino acids in a dominant immunogen of Plasmodium falciparum. Proc Natl Acad Sci U S A. 1985; 82(2):543-7. PMC: 397076. DOI: 10.1073/pnas.82.2.543. View

18.

Lawrence F . Induction of heat shock and stress proteins in promastigotes of three Leishmania species. Proc Natl Acad Sci U S A. 1985; 82(13):4414-7. PMC: 391111. DOI: 10.1073/pnas.82.13.4414. View

19.

Van der Ploeg L, Giannini S, Cantor C . Heat shock genes: regulatory role for differentiation in parasitic protozoa. Science. 1985; 228(4706):1443-6. DOI: 10.1126/science.4012301. View

20.

Brown G, Culvenor J, Crewther P, Bianco A, Coppel R, Saint R . Localization of the ring-infected erythrocyte surface antigen (RESA) of Plasmodium falciparum in merozoites and ring-infected erythrocytes. J Exp Med. 1985; 162(2):774-9. PMC: 2187736. DOI: 10.1084/jem.162.2.774. View