Drosophila Gene Related to the Major Heat Shock-induced Gene is Transcribed at Normal Temperatures and Not Induced by Heat Shock

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1982 Jan 1

PMID 6804941

Citations 75

Authors

T D Ingolia

E A Craig

Affiliations

Soon will be listed here.

Abstract

A gene related to the major heat shock-induced (hsp70) gene of Drosophila has been isolated from the sibling species D. melanogaster and D. simulans. This heat shock-cognate (hsc70) gene is present at cytological locus 70C. The primary sequence of approximately one-third of the protein-coding region has been compared with that of the hsp70 gene; 72% homology of the base sequence and 74% homology of the deduced amino acid sequence was found. In the codon specifying amino acid 66, the hsc70 gene contains an insertion of 1.7 kilobases; the hsp70 genes contain no intervening sequences. The sequence at the 5' and 3' junctions of the insertion is similar to that found in many intervening sequences. cDNA extension experiments indicate that the hsc70 gene is transcribed at normal temperatures in adult flies and that transcription is not enhanced by heat treatment.

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References

Ashburner M . Patterns of puffing activity in the salivary gland chromosomes of Drosophila. V. Responses to environmental treatments. Chromosoma. 1970; 31(3):356-76. DOI: 10.1007/BF00321231. View

Spradling A, Penman S, Pardue M . Analysis of drosophila mRNA by in situ hybridization: sequences transcribed in normal and heat shocked cultured cells. Cell. 1975; 4(4):395-404. DOI: 10.1016/0092-8674(75)90160-9. View

McKenzie S, Henikoff S, Meselson M . Localization of RNA from heat-induced polysomes at puff sites in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1975; 72(3):1117-21. PMC: 432477. DOI: 10.1073/pnas.72.3.1117. View

Bonner J, Pardue M . The effect of heat shock on RNA synthesis in Drosophila tissues. Cell. 1976; 8(1):43-50. DOI: 10.1016/0092-8674(76)90183-5. View

Spradling A, Pardue M, Penman S . Messenger RNA in heat-shocked Drosophila cells. J Mol Biol. 1977; 109(4):559-87. DOI: 10.1016/s0022-2836(77)80091-0. View

Henikoff S, Meselson M . Transcription at two heat shock loci in Drosophila. Cell. 1977; 12(2):441-51. DOI: 10.1016/0092-8674(77)90120-9. View

Ish-Horowicz D, Holden J, Gehring W . Deletions of two heat-activated loci in Drosophila melanogaster and their effects on heat-induced protein synthesis. Cell. 1977; 12(3):643-52. DOI: 10.1016/0092-8674(77)90264-1. View

Kelley P, SCHLESINGER M . The effect of amino acid analogues and heat shock on gene expression in chicken embryo fibroblasts. Cell. 1978; 15(4):1277-86. DOI: 10.1016/0092-8674(78)90053-3. View

Craig E, McCarthy B, Wadsworth S . Sequence organization of two recombinant plasmids containing genes for the major heat shock-induced protein of D. melanogaster. Cell. 1979; 16(3):575-88. DOI: 10.1016/0092-8674(79)90031-x. View

10.

Miller M, Xuong N, Geiduschek E . A response of protein synthesis to temperature shift in the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1979; 76(10):5222-5. PMC: 413112. DOI: 10.1073/pnas.76.10.5222. View

11.

Chirgwin J, Przybyla A, MacDonald R, Rutter W . Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979; 18(24):5294-9. DOI: 10.1021/bi00591a005. View

12.

Ish-Horowicz D, Pinchin S, Schedl P, Artavanis-Tsakonas S, Mirault M . Genetic and molecular analysis of the 87A7 and 87C1 heat-inducible loci of D. melanogaster. Cell. 1979; 18(4):1351-8. DOI: 10.1016/0092-8674(79)90245-9. View

13.

Nishioka Y, Leder A, Leder P . Unusual alpha-globin-like gene that has cleanly lost both globin intervening sequences. Proc Natl Acad Sci U S A. 1980; 77(5):2806-9. PMC: 349493. DOI: 10.1073/pnas.77.5.2806. View

14.

Arrigo A, Fakan S, TISSIERES A . Localization of the heat shock-induced proteins in Drosophila melanogaster tissue culture cells. Dev Biol. 1980; 78(1):86-103. DOI: 10.1016/0012-1606(80)90320-6. View

15.

Vanin E, Goldberg G, Tucker P, SMITHIES O . A mouse alpha-globin-related pseudogene lacking intervening sequences. Nature. 1980; 286(5770):222-6. DOI: 10.1038/286222a0. View

16.

Velazquez J, DiDomenico B, Lindquist S . Intracellular localization of heat shock proteins in Drosophila. Cell. 1980; 20(3):679-89. DOI: 10.1016/0092-8674(80)90314-1. View

17.

Loomis W, Wheeler S . Heat shock response of Dictyostelium. Dev Biol. 1980; 79(2):399-408. DOI: 10.1016/0012-1606(80)90125-6. View

18.

Corces V, Holmgren R, Freund R, Morimoto R, Meselson M . Four heat shock proteins of Drosophila melanogaster coded within a 12-kilobase region in chromosome subdivision 67B. Proc Natl Acad Sci U S A. 1980; 77(9):5390-3. PMC: 350064. DOI: 10.1073/pnas.77.9.5390. View

19.

Ingolia T, Craig E, McCarthy B . Sequence of three copies of the gene for the major Drosophila heat shock induced protein and their flanking regions. Cell. 1980; 21(3):669-79. DOI: 10.1016/0092-8674(80)90430-4. View

20.

Torok I, Karch F . Nucleotide sequences of heat shock activated genes in Drosophila melanogaster. I. Sequences in the regions of the 5' and 3' ends of the hsp 70 gene in the hybrid plasmid 56H8. Nucleic Acids Res. 1980; 8(14):3105-23. PMC: 324358. DOI: 10.1093/nar/8.14.3105. View