Machine-Driven Enzymatic Oligosaccharide Synthesis by Using A Peptide Synthesizer

Overview

Journal Angew Chem Int Ed Engl

Specialty Chemistry

Date 2018 Oct 31

PMID 30375138

Citations 28

Authors

Jiabin Zhang

Congcong Chen

Madhusudhan Reddy Gadi

Christopher Gibbons

Yuxi Guo

Xuefeng Cao

Garrett Edmunds

Shuaishuai Wang

Ding Liu

Jin Yu

Liuqing Wen

Peng G Wang

Affiliations

Soon will be listed here.

Abstract

For decades, researchers have endeavored to develop a general automated system to synthesize oligosaccharides that is comparable to the preparation of oligonucleotides and oligopeptides by commercially available machines. Inspired by the success of automated oligosaccharide synthesis through chemical glycosylation, a fully automated system is reported for oligosaccharides synthesis through enzymatic glycosylation in aqueous solution. The designed system is based on the use of a thermosensitive polymer and a commercially available peptide synthesizer. This study represents a proof-of-concept demonstration that the enzymatic synthesis of oligosaccharides can be achieved in an automated manner using a commercially available peptide synthesizer.

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