» Articles » PMID: 30286703

Ergothioneine Production with Aspergillus Oryzae

Overview
Date 2018 Oct 6
PMID 30286703
Citations 18
Authors
Affiliations
Soon will be listed here.
Abstract

To establish a reliable and practical ergothioneine (ERG) supply, we employed fermentative ERG production using Aspergillus oryzae, a fungus used for food production. We heterologously overexpressed the egt-1 and -2 genes of Neurospora crassa in A. oryzae and succeeded in producing ERG (231.0 mg/kg of media, which was 20 times higher than the wild type). Abbreviations: ERG: ergothioneine; HER: hercynine; Cys-HER: hercynylcysteine-sulfoxide; SAM: S-adenosylmethionine; SAH: S-adenosylhomocysteine; l-His: l-histidine; l-Cys: l-cysteine; LC-ESI-MS: liquid chromatography-electrospray ionization-mass spectrometry.

Citing Articles

Ergothioneine biosynthesis: The present state and future prospect.

Liang L, Shan-Shan X, Yan-Jun J Synth Syst Biotechnol. 2024; 10(1):314-325.

PMID: 39717282 PMC: 11664081. DOI: 10.1016/j.synbio.2024.10.008.


Advancing Industry: Gene Manipulation and Sustainable Biotechnological Strategies.

Hu Y, Wu Y, Song J, Ma M, Xiao Y, Zeng B Bioengineering (Basel). 2024; 11(8).

PMID: 39199741 PMC: 11351413. DOI: 10.3390/bioengineering11080783.


Flavor assessment of a lactic fermented vinegar described in Japanese books from the Edo period (1603-1867).

Yanagihara N, Mayumi M, Yoshikawa J, Akuzawa S, Fujii A, Nagano M Heliyon. 2024; 10(11):e32344.

PMID: 38961972 PMC: 11219324. DOI: 10.1016/j.heliyon.2024.e32344.


Strategies for the Enhancement of Secondary Metabolite Production via Biosynthesis Gene Cluster Regulation in .

Jia X, Song J, Wu Y, Feng S, Sun Z, Hu Y J Fungi (Basel). 2024; 10(5).

PMID: 38786667 PMC: 11121810. DOI: 10.3390/jof10050312.


Edible mycelium bioengineered for enhanced nutritional value and sensory appeal using a modular synthetic biology toolkit.

Maini Rekdal V, van der Luijt C, Chen Y, Kakumanu R, Baidoo E, Petzold C Nat Commun. 2024; 15(1):2099.

PMID: 38485948 PMC: 10940619. DOI: 10.1038/s41467-024-46314-8.