Association of Vitamin D Receptor Gene Polymorphism (VDR) with Vitamin D Deficiency, Metabolic and Inflammatory Markers in Egyptian Obese Women

Overview

Journal Genes Dis

Date 2018 Sep 28

PMID 30258921

Citations 25

Authors

Moushira Zaki

Sanaa Kamal

Walaa A Basha

Eman Youness

Wafaa Ezzat

Hala El-Bassyouni

Khalda Amr

Affiliations

Soon will be listed here.

Abstract

Vitamin D deficiency might contribute to the pathogenesis of metabolic syndrome and could cause immune disturbance. The aim of this study is to analyze the associations between Vitamin D receptor (VDR) gene polymorphism, serum 25-hydroxy vitamin D, metabolic and inflammatory biomarkers in Egyptian obese women. The study included 201 obese women with vitamin D deficiency and 249 obese matched age healthy controls with sufficient vitamin D levels. Their age ranged between 25 and 35 years. Inflammatory biomarkers (interleukin-6 and C-reactive protein) and serum 25(OH) D were measured by enzyme-linked immunosorbent assay. Insulin resistance (IR) was determined by the homeostasis model assessment of insulin resistance (HOMA-IR).Vitamin D receptor (VDR) gene polymorphisms of FokI, ApaI, and TaqI were studied by PCR using the restriction fragment length polymorphism (RFLP) technique. Obese women with vitamin D deficiency had significant higher values of inflammatory and metabolic parameters compared to controls. Multivariable-logistic regression showed associations between 25(OH) D deficiency and metabolic components when comparing cases with controls. Moreover, cases carrying polymorphic alleles showed significant lower levels of serum 25(OH) D and higher HOMA-IR, blood pressure levels and lipid parameters compared to those with the wild type homozygote in obese cases with vitamin D deficiency. Vitamin D deficiency in Egyptian obese women with vitamin D deficiency is associated with abnormal metabolic components and abnormal inflammatory biomarkers. Moreover, VDR polymorphisms play important role in immune and inflammation status.

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References

Ju S, Jeong H, Kim D . Blood vitamin D status and metabolic syndrome in the general adult population: a dose-response meta-analysis. J Clin Endocrinol Metab. 2014; 99(3):1053-63. DOI: 10.1210/jc.2013-3577. View

Speer G, Cseh K, Winkler G, Vargha P, Braun E, Takacs I . Vitamin D and estrogen receptor gene polymorphisms in type 2 diabetes mellitus and in android type obesity. Eur J Endocrinol. 2001; 144(4):385-9. DOI: 10.1530/eje.0.1440385. View

Alvarez J, Ashraf A . Role of vitamin d in insulin secretion and insulin sensitivity for glucose homeostasis. Int J Endocrinol. 2009; 2010:351385. PMC: 2778451. DOI: 10.1155/2010/351385. View

Mackawy A, Badawi M . Association of vitamin D and vitamin D receptor gene polymorphisms with chronic inflammation, insulin resistance and metabolic syndrome components in type 2 diabetic Egyptian patients. Meta Gene. 2015; 2:540-56. PMC: 4287888. DOI: 10.1016/j.mgene.2014.07.002. View

Palacios C, Gonzalez L . Is vitamin D deficiency a major global public health problem?. J Steroid Biochem Mol Biol. 2013; 144 Pt A:138-45. PMC: 4018438. DOI: 10.1016/j.jsbmb.2013.11.003. View

Ye W, Reis A, Dubois-Laforgue D, Bellanne-Chantelot C, Timsit J, Velho G . Vitamin D receptor gene polymorphisms are associated with obesity in type 2 diabetic subjects with early age of onset. Eur J Endocrinol. 2001; 145(2):181-6. DOI: 10.1530/eje.0.1450181. View

Forouhi N, Luan J, Cooper A, Boucher B, Wareham N . Baseline serum 25-hydroxy vitamin d is predictive of future glycemic status and insulin resistance: the Medical Research Council Ely Prospective Study 1990-2000. Diabetes. 2008; 57(10):2619-25. PMC: 2551670. DOI: 10.2337/db08-0593. View

Ginde A, Liu M, Camargo Jr C . Demographic differences and trends of vitamin D insufficiency in the US population, 1988-2004. Arch Intern Med. 2009; 169(6):626-32. PMC: 3447083. DOI: 10.1001/archinternmed.2008.604. View

Filus A, Trzmiel A, Kuliczkowska-Plaksej J, Tworowska U, Jedrzejuk D, Milewicz A . Relationship between vitamin D receptor BsmI and FokI polymorphisms and anthropometric and biochemical parameters describing metabolic syndrome. Aging Male. 2008; 11(3):134-9. DOI: 10.1080/13685530802273426. View

10.

Hilger J, Friedel A, Herr R, Rausch T, Roos F, Wahl D . A systematic review of vitamin D status in populations worldwide. Br J Nutr. 2013; 111(1):23-45. DOI: 10.1017/S0007114513001840. View

11.

Wortsman J, Matsuoka L, Chen T, Lu Z, Holick M . Decreased bioavailability of vitamin D in obesity. Am J Clin Nutr. 2000; 72(3):690-3. DOI: 10.1093/ajcn/72.3.690. View

12.

Tai K, Need A, Horowitz M, Chapman I . Vitamin D, glucose, insulin, and insulin sensitivity. Nutrition. 2008; 24(3):279-85. DOI: 10.1016/j.nut.2007.11.006. View

13.

Arabi A, El Rassi R, Fuleihan G . Hypovitaminosis D in developing countries-prevalence, risk factors and outcomes. Nat Rev Endocrinol. 2010; 6(10):550-61. DOI: 10.1038/nrendo.2010.146. View

14.

Chiu K, Chu A, Go V, Saad M . Hypovitaminosis D is associated with insulin resistance and beta cell dysfunction. Am J Clin Nutr. 2004; 79(5):820-5. DOI: 10.1093/ajcn/79.5.820. View

15.

Ortlepp J, Lauscher J, Hoffmann R, Hanrath P, Joost H . The vitamin D receptor gene variant is associated with the prevalence of type 2 diabetes mellitus and coronary artery disease. Diabet Med. 2001; 18(10):842-5. DOI: 10.1046/j.1464-5491.2001.00585.x. View

16.

Miller M, Harrison R, Wlodawer A, Appella E, Sussman J . Crystal structure of 15-mer DNA duplex containing unpaired bases. Nature. 1988; 334(6177):85-6. DOI: 10.1038/334085a0. View

17.

Holick M, Binkley N, Bischoff-Ferrari H, Gordon C, Hanley D, Heaney R . Evaluation, treatment, and prevention of vitamin D deficiency: an Endocrine Society clinical practice guideline. J Clin Endocrinol Metab. 2011; 96(7):1911-30. DOI: 10.1210/jc.2011-0385. View

18.

Bid H, Konwar R, Aggarwal C, Gautam S, Saxena M, Nayak V . Vitamin D receptor (FokI, BsmI and TaqI) gene polymorphisms and type 2 diabetes mellitus: a North Indian study. Indian J Med Sci. 2009; 63(5):187-94. View

19.

Vimaleswaran K, Berry D, Lu C, Tikkanen E, Pilz S, Hiraki L . Causal relationship between obesity and vitamin D status: bi-directional Mendelian randomization analysis of multiple cohorts. PLoS Med. 2013; 10(2):e1001383. PMC: 3564800. DOI: 10.1371/journal.pmed.1001383. View

20.

Naito M, Miyaki K, Naito T, Zhang L, Hoshi K, Hara A . Association between vitamin D receptor gene haplotypes and chronic periodontitis among Japanese men. Int J Med Sci. 2007; 4(4):216-22. PMC: 1975778. DOI: 10.7150/ijms.4.216. View