Crystal Structure of 15-mer DNA Duplex Containing Unpaired Bases

Overview

Journal Nature

Specialty Science

Date 1988 Jul 7

PMID 3386752

Citations 13

Authors

M Miller

R W Harrison

A Wlodawer

E Appella

J L Sussman

Affiliations

Soon will be listed here.

Abstract

Errors during DNA replication or repair can lead to the presence of unpaired or inserted bases in the double helix, as well as to mismatched base pairs. So far only structures of the latter type have been characterized by X-ray crystallography. We report here a 3-A crystal structure of DNA 15-mer d(CGCGAAATTTACGCG), which forms a duplex with two unpaired adenine residues looped outside the B-type helix. This arrangement is in disagreement with the nuclear magnetic resonance spectroscopy results for the same 15-mer in solution, indicating polymorphic nature of the structure adopted by this sequence.

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