Effects of Different Doses of Fructooligosaccharides (FOS) on the Composition of Mice Fecal Microbiota, Especially the Bifidobacterium Composition

Overview

Journal Nutrients

Date 2018 Aug 18

PMID 30115879

Citations 37

Authors

Bingyong Mao

Jiayu Gu

Dongyao Li

Shumao Cui

Jianxin Zhao

Hao Zhang

Wei Chen

Affiliations

Soon will be listed here.

Abstract

Fructooligosaccharides (FOS) are a well-known class of prebiotic and are considered to selectively stimulate the growth of bifidobacteria in the gut. Previous studies focused on the growth stimulation of , but they did not further investigate the bifidobacterial composition and the specific species that were stimulated. In this study, mice were fed with FOS in different doses for four weeks and the composition of fecal microbiota, in particular , was analyzed by sequencing the V3⁻V4 region and the gene on the MiSeq platform, respectively. In the high-dose group, the relative abundance of Actinobacteria was significantly increased, which was mainly contributed by . At the genus level, the relative abundances of and were also significantly increased. Through the sequencing, 14 species of were identified, among which was most abundant. After FOS treatment, became almost the sole bifidobacterial species (>95%). strains were isolated and demonstrated their ability to metabolize FOS by high performance liquid chromatography (HPLC). Therefore, we inferred that FOS significantly stimulated the growth of in mice. Further investigations are needed to reveal the mechanism of selectiveness between FOS and , which would aid our understanding of the basic principles between dietary carbohydrates and host health.

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