In Vitro Fermentation of Sugar Beet Arabino-oligosaccharides by Fecal Microbiota Obtained from Patients with Ulcerative Colitis to Selectively Stimulate the Growth of Bifidobacterium Spp. and Lactobacillus Spp

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2011 Oct 11

PMID 21984234

Citations 22

Authors

Louise Kristine Vigsnaes

Jesper Holck

Anne S Meyer

Tine Rask Licht

Affiliations

Soon will be listed here.

Abstract

The potential prebiotic properties of arabino-oligosaccharides (AOS) derived from sugar beet pulp was studied using mixed cultures of human fecal bacteria from patients with ulcerative colitis (UC), in remission or with active disease, and in healthy controls. These results were compared to those for fructo-oligosaccharides (FOS), which are known to have a prebiotic effect. Fermentation studies were carried out using a small-scale static batch system, and changes in the fecal microbial communities and metabolites were monitored after 24 h by quantitative real-time PCR and short-chain fatty acid analysis. With a few minor exceptions, AOS affected the communities similarly to what was seen for FOS. Quantitative real-time PCR revealed that Bifidobacterium spp. and Lactobacillus spp. were selectively increased after fermentation of AOS or FOS by fecal microbiota derived from UC patients. The stimulation of growth of Lactobacillus spp. and Bifidobacterium spp. was accompanied by a high production of acetate and hence a decrease of pH. The fermentation of AOS may help improve the inflammatory conditions in UC patients through stimulation of bacteria eliciting anti-inflammatory responses and through production of acetate. AOS may therefore represent a new prebiotic candidate for reduction of the risk of flare-ups in UC patients. However, human trials are needed to confirm a health-promoting effect.

Citing Articles

Exo- and Endo-1,5-α-L-Arabinanases and Prebiotic Arabino-Oligosaccharides Production.

Ju Y, Im S, Jung D, Son M, Park C, Jeon M J Microbiol Biotechnol. 2025; 35:e2412052.

PMID: 39849927 PMC: 11813348. DOI: 10.4014/jmb.2412.12052.

Functional Characterization of Endo- and Exo-Hydrolase Genes in Arabinan Degradation Gene Cluster of subsp. .

Kang Y, Choi C, Kang J, Ju Y, Kim H, Han N Int J Mol Sci. 2024; 25(6).

PMID: 38542148 PMC: 10970622. DOI: 10.3390/ijms25063175.

Effect of Sugar Beet Pulp on the Composition and Predicted Function of Equine Fecal Microbiota.

Ford T, McAdams Z, Townsend K, Martin L, Johnson P, Ericsson A Biology (Basel). 2023; 12(9).

PMID: 37759653 PMC: 10525916. DOI: 10.3390/biology12091254.

The Potential of Pectins to Modulate the Human Gut Microbiota Evaluated by In Vitro Fermentation: A Systematic Review.

Pascale N, Gu F, Larsen N, Jespersen L, Respondek F Nutrients. 2022; 14(17).

PMID: 36079886 PMC: 9460662. DOI: 10.3390/nu14173629.

Cranberry Arabino-Xyloglucan and Pectic Oligosaccharides Induce Growth and Short-Chain Fatty Acid Production.

Hotchkiss Jr A, Renye Jr J, White A, Nunez A, Guron G, Chau H Microorganisms. 2022; 10(7).

PMID: 35889065 PMC: 9319371. DOI: 10.3390/microorganisms10071346.

References

Hart A, Al-Hassi H, Rigby R, Bell S, Emmanuel A, Knight S . Characteristics of intestinal dendritic cells in inflammatory bowel diseases. Gastroenterology. 2005; 129(1):50-65. DOI: 10.1053/j.gastro.2005.05.013. View

Gibson G . Dietary modulation of the human gut microflora using the prebiotics oligofructose and inulin. J Nutr. 1999; 129(7 Suppl):1438S-41S. DOI: 10.1093/jn/129.7.1438S. View

Mandalari G, Nueno Palop C, Tuohy K, Gibson G, Bennett R, Waldron K . In vitro evaluation of the prebiotic activity of a pectic oligosaccharide-rich extract enzymatically derived from bergamot peel. Appl Microbiol Biotechnol. 2006; 73(5):1173-9. DOI: 10.1007/s00253-006-0561-9. View

Jacobs D, Gaudier E, van Duynhoven J, Vaughan E . Non-digestible food ingredients, colonic microbiota and the impact on gut health and immunity: a role for metabolomics. Curr Drug Metab. 2009; 10(1):41-54. DOI: 10.2174/138920009787048383. View

Lara-Villoslada F, de Haro O, Camuesco D, Comalada M, Velasco J, Zarzuelo A . Short-chain fructooligosaccharides, in spite of being fermented in the upper part of the large intestine, have anti-inflammatory activity in the TNBS model of colitis. Eur J Nutr. 2006; 45(7):418-25. DOI: 10.1007/s00394-006-0610-2. View

Oosterveld A, Beldman G, Schols H, Voragen A . Characterization of arabinose and ferulic acid rich pectic polysaccharides and hemicelluloses from sugar beet pulp. Carbohydr Res. 2000; 328(2):185-97. DOI: 10.1016/s0008-6215(00)00095-1. View

Andrews C, Griffiths T, Kaufman J, Vergnolle N, Surette M, Rioux K . Mesalazine (5-aminosalicylic acid) alters faecal bacterial profiles, but not mucosal proteolytic activity in diarrhoea-predominant irritable bowel syndrome. Aliment Pharmacol Ther. 2011; 34(3):374-83. DOI: 10.1111/j.1365-2036.2011.04732.x. View

Ruemmele F, Schwartz S, Seidman E, Dionne S, Levy E, Lentze M . Butyrate induced Caco-2 cell apoptosis is mediated via the mitochondrial pathway. Gut. 2002; 52(1):94-100. PMC: 1773532. DOI: 10.1136/gut.52.1.94. View

Ruas-Madiedo P, Medrano M, Salazar N, G de Los Reyes-Gavilan C, Perez P, Abraham A . Exopolysaccharides produced by Lactobacillus and Bifidobacterium strains abrogate in vitro the cytotoxic effect of bacterial toxins on eukaryotic cells. J Appl Microbiol. 2010; 109(6):2079-86. DOI: 10.1111/j.1365-2672.2010.04839.x. View

10.

De Preter V, Hamer H, Windey K, Verbeke K . The impact of pre- and/or probiotics on human colonic metabolism: does it affect human health?. Mol Nutr Food Res. 2011; 55(1):46-57. DOI: 10.1002/mnfr.201000451. View

11.

Rowan F, Docherty N, Murphy M, Murphy B, Coffey J, OConnell P . Desulfovibrio bacterial species are increased in ulcerative colitis. Dis Colon Rectum. 2010; 53(11):1530-6. DOI: 10.1007/DCR.0b013e3181f1e620. View

12.

Thomassen L, Vigsnaes L, Licht T, Mikkelsen J, Meyer A . Maximal release of highly bifidogenic soluble dietary fibers from industrial potato pulp by minimal enzymatic treatment. Appl Microbiol Biotechnol. 2011; 90(3):873-84. DOI: 10.1007/s00253-011-3092-y. View

13.

Delroisse J, Boulvin A, Parmentier I, Dauphin R, Vandenbol M, Portetelle D . Quantification of Bifidobacterium spp. and Lactobacillus spp. in rat fecal samples by real-time PCR. Microbiol Res. 2009; 163(6):663-70. DOI: 10.1016/j.micres.2006.09.004. View

14.

Palframan R, Gibson G, Rastall R . Effect of pH and dose on the growth of gut bacteria on prebiotic carbohydrates in vitro. Anaerobe. 2006; 8(5):287-92. DOI: 10.1006/anae.2002.0434. View

15.

Zocco M, Zileri Dal Verme L, Cremonini F, Piscaglia A, Nista E, Candelli M . Efficacy of Lactobacillus GG in maintaining remission of ulcerative colitis. Aliment Pharmacol Ther. 2006; 23(11):1567-74. DOI: 10.1111/j.1365-2036.2006.02927.x. View

16.

Christl S, Eisner H, Dusel G, Kasper H, Scheppach W . Antagonistic effects of sulfide and butyrate on proliferation of colonic mucosa: a potential role for these agents in the pathogenesis of ulcerative colitis. Dig Dis Sci. 1996; 41(12):2477-81. DOI: 10.1007/BF02100146. View

17.

Shen J, Zhang B, Wei G, Pang X, Wei H, Li M . Molecular profiling of the Clostridium leptum subgroup in human fecal microflora by PCR-denaturing gradient gel electrophoresis and clone library analysis. Appl Environ Microbiol. 2006; 72(8):5232-8. PMC: 1538713. DOI: 10.1128/AEM.00151-06. View

18.

Rycroft C, Jones M, Gibson G, Rastall R . Fermentation properties of gentio-oligosaccharides. Lett Appl Microbiol. 2001; 32(3):156-61. DOI: 10.1046/j.1472-765x.2001.00875.x. View

19.

Binder V . A comparison between clinical state, macroscopic and microscopic appearances of rectal mucosa, and cytologic picture of mucosal exudate in ulcerative colitis. Scand J Gastroenterol. 1970; 5(7):627-32. View

20.

Holck J, Lorentzen A, Vigsnaes L, Licht T, Mikkelsen J, Meyer A . Feruloylated and nonferuloylated arabino-oligosaccharides from sugar beet pectin selectively stimulate the growth of Bifidobacterium spp. in human fecal in vitro fermentations. J Agric Food Chem. 2011; 59(12):6511-9. DOI: 10.1021/jf200996h. View