Regional Diversity in the Postsynaptic Proteome of the Mouse Brain

Overview

Journal Proteomes

Date 2018 Aug 4

PMID 30071621

Citations 23

Authors

Marcia Roy

Oksana Sorokina

Colin McLean

Silvia Tapia-Gonzalez

Javier DeFelipe

J Douglas Armstrong

Seth G N Grant

Affiliations

Soon will be listed here.

Abstract

The proteome of the postsynaptic terminal of excitatory synapses comprises over one thousand proteins in vertebrate species and plays a central role in behavior and brain disease. The brain is organized into anatomically distinct regions and whether the synapse proteome differs across these regions is poorly understood. Postsynaptic proteomes were isolated from seven forebrain and hindbrain regions in mice and their composition determined using proteomic mass spectrometry. Seventy-four percent of proteins showed differential expression and each region displayed a unique compositional signature. These signatures correlated with the anatomical divisions of the brain and their embryological origins. Biochemical pathways controlling plasticity and disease, protein interaction networks and individual proteins involved with cognition all showed differential regional expression. Combining proteomic and connectomic data shows that interconnected regions have specific proteome signatures. Diversity in synapse proteome composition is key feature of mouse and human brain structure.

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