SINC-seq: Correlation of Transient Gene Expressions Between Nucleus and Cytoplasm Reflects Single-cell Physiology

Overview

Journal Genome Biol

Specialties Biology
Genetics

Date 2018 Jun 7

PMID 29871653

Citations 24

Authors

Mahmoud N Abdelmoez

Kei Iida

Yusuke Oguchi

Hidekazu Nishikii

Ryuji Yokokawa

Hidetoshi Kotera

Sotaro Uemura

Juan G Santiago

Hirofumi Shintaku

Affiliations

Soon will be listed here.

Abstract

We report a microfluidic system that physically separates nuclear RNA (nucRNA) and cytoplasmic RNA (cytRNA) from a single cell and enables single-cell integrated nucRNA and cytRNA-sequencing (SINC-seq). SINC-seq constructs two individual RNA-seq libraries, nucRNA and cytRNA, per cell, quantifies gene expression in the subcellular compartments, and combines them to create novel single-cell RNA-seq data. Leveraging SINC-seq, we discover distinct natures of correlation among cytRNA and nucRNA that reflect the transient physiological state of single cells. These data provide unique insights into the regulatory network of messenger RNA from the nucleus toward the cytoplasm at the single-cell level.

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