Genetic and Biochemical Characterization of OXA-519, a Novel OXA-48-Like β-Lactamase

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 2018 Jun 6

PMID 29866857

Citations 18

Authors

Laura Dabos

Pierre Bogaerts

Remy A Bonnin

Agustin Zavala

Pierre Sacre

Bogdan I Iorga

Daniel T Huang

Youri Glupczynski

Thierry Naas

Affiliations

Soon will be listed here.

Abstract

A multidrug-resistant 1210 isolate with reduced carbapenem susceptibility revealed the presence of a novel plasmid-encoded gene, named The 60.7-kb plasmid (pOXA-519) was similar to the IncL-OXA-48 prototypical plasmid except for a ca. 2-kb deletion due to an IS insertion. OXA-519 differed from OXA-48 by a Val120Leu substitution, which resulted in an overall reduced β-lactam-hydrolysis profile, except those for ertapenem and meropenem, which were increased. Thus, detection of OXA-519 producers using biochemical tests that monitor imipenem hydrolysis will be difficult.

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References

Boutal H, Vogel A, Bernabeu S, Devilliers K, Creton E, Cotellon G . A multiplex lateral flow immunoassay for the rapid identification of NDM-, KPC-, IMP- and VIM-type and OXA-48-like carbapenemase-producing Enterobacteriaceae. J Antimicrob Chemother. 2018; 73(4):909-915. PMC: 5890661. DOI: 10.1093/jac/dkx521. View

Bogaerts P, Yunus S, Massart M, Huang T, Glupczynski Y . Evaluation of the BYG Carba Test, a New Electrochemical Assay for Rapid Laboratory Detection of Carbapenemase-Producing Enterobacteriaceae. J Clin Microbiol. 2015; 54(2):349-58. PMC: 4733164. DOI: 10.1128/JCM.02404-15. View

Naas T, Oueslati S, Bonnin R, Dabos M, Zavala A, Dortet L . Beta-lactamase database (BLDB) - structure and function. J Enzyme Inhib Med Chem. 2017; 32(1):917-919. PMC: 6445328. DOI: 10.1080/14756366.2017.1344235. View

Rodrigues C, Bavlovic J, Machado E, Amorim J, Peixe L, Novais A . KPC-3-Producing Klebsiella pneumoniae in Portugal Linked to Previously Circulating Non-CG258 Lineages and Uncommon Genetic Platforms (Tn4401d-IncFIA and Tn4401d-IncN). Front Microbiol. 2016; 7:1000. PMC: 4923139. DOI: 10.3389/fmicb.2016.01000. View

Potron A, Rondinaud E, Poirel L, Belmonte O, Boyer S, Camiade S . Genetic and biochemical characterisation of OXA-232, a carbapenem-hydrolysing class D β-lactamase from Enterobacteriaceae. Int J Antimicrob Agents. 2013; 41(4):325-9. DOI: 10.1016/j.ijantimicag.2012.11.007. View

Poirel L, Bonnin R, Nordmann P . Genetic support and diversity of acquired extended-spectrum β-lactamases in Gram-negative rods. Infect Genet Evol. 2012; 12(5):883-93. DOI: 10.1016/j.meegid.2012.02.008. View

Potron A, Nordmann P, Lafeuille E, Maskari Z, Al Rashdi F, Poirel L . Characterization of OXA-181, a carbapenem-hydrolyzing class D beta-lactamase from Klebsiella pneumoniae. Antimicrob Agents Chemother. 2011; 55(10):4896-9. PMC: 3186949. DOI: 10.1128/AAC.00481-11. View

Aubert D, Naas T, Heritier C, Poirel L, Nordmann P . Functional characterization of IS1999, an IS4 family element involved in mobilization and expression of beta-lactam resistance genes. J Bacteriol. 2006; 188(18):6506-14. PMC: 1595497. DOI: 10.1128/JB.00375-06. View

Fabrega A, Madurga S, Giralt E, Vila J . Mechanism of action of and resistance to quinolones. Microb Biotechnol. 2011; 2(1):40-61. PMC: 3815421. DOI: 10.1111/j.1751-7915.2008.00063.x. View

10.

Zankari E, Hasman H, Cosentino S, Vestergaard M, Rasmussen S, Lund O . Identification of acquired antimicrobial resistance genes. J Antimicrob Chemother. 2012; 67(11):2640-4. PMC: 3468078. DOI: 10.1093/jac/dks261. View

11.

Oueslati S, Nordmann P, Poirel L . Heterogeneous hydrolytic features for OXA-48-like β-lactamases. J Antimicrob Chemother. 2015; 70(4):1059-63. DOI: 10.1093/jac/dku524. View

12.

Larsen M, Cosentino S, Rasmussen S, Friis C, Hasman H, Marvig R . Multilocus sequence typing of total-genome-sequenced bacteria. J Clin Microbiol. 2012; 50(4):1355-61. PMC: 3318499. DOI: 10.1128/JCM.06094-11. View

13.

Jousset A, Dabos L, Bonnin R, Girlich D, Potron A, Cabanel N . CTX-M-15-Producing Shewanella Species Clinical Isolate Expressing OXA-535, a Chromosome-Encoded OXA-48 Variant, Putative Progenitor of the Plasmid-Encoded OXA-436. Antimicrob Agents Chemother. 2017; 62(1). PMC: 5740354. DOI: 10.1128/AAC.01879-17. View

14.

Bernabeu S, Dortet L, Naas T . Evaluation of the β-CARBA™ test, a colorimetric test for the rapid detection of carbapenemase activity in Gram-negative bacilli. J Antimicrob Chemother. 2017; 72(6):1646-1658. DOI: 10.1093/jac/dkx061. View

15.

Lamotte-Brasseur J, Knox J, Kelly J, Charlier P, Fonze E, Dideberg O . The structures and catalytic mechanisms of active-site serine beta-lactamases. Biotechnol Genet Eng Rev. 1994; 12:189-230. DOI: 10.1080/02648725.1994.10647912. View

16.

Zhou K, Lokate M, Deurenberg R, Tepper M, Arends J, Raangs E . Use of whole-genome sequencing to trace, control and characterize the regional expansion of extended-spectrum β-lactamase producing ST15 Klebsiella pneumoniae. Sci Rep. 2016; 6:20840. PMC: 4749987. DOI: 10.1038/srep20840. View

17.

Ewers C, Stamm I, Pfeifer Y, Wieler L, Kopp P, Schonning K . Clonal spread of highly successful ST15-CTX-M-15 Klebsiella pneumoniae in companion animals and horses. J Antimicrob Chemother. 2014; 69(10):2676-80. DOI: 10.1093/jac/dku217. View

18.

Potron A, Poirel L, Nordmann P . Derepressed transfer properties leading to the efficient spread of the plasmid encoding carbapenemase OXA-48. Antimicrob Agents Chemother. 2013; 58(1):467-71. PMC: 3910765. DOI: 10.1128/AAC.01344-13. View

19.

Smith C, Antunes N, Stewart N, Frase H, Toth M, Kantardjieff K . Structural Basis for Enhancement of Carbapenemase Activity in the OXA-51 Family of Class D β-Lactamases. ACS Chem Biol. 2015; 10(8):1791-6. PMC: 4546549. DOI: 10.1021/acschembio.5b00090. View

20.

Poirel L, Bonnin R, Nordmann P . Genetic features of the widespread plasmid coding for the carbapenemase OXA-48. Antimicrob Agents Chemother. 2011; 56(1):559-62. PMC: 3256075. DOI: 10.1128/AAC.05289-11. View