Evaluation of Point Mutation Carbapenemase-producing in Prapokklao Hospital, Thailand

Overview

Journal Microbiol Spectr

Specialty Microbiology

Date 2024 Oct 17

PMID 39417625

Authors

Sirijan Santajit

Witawat Tunyong

Thida Kong-Ngoen

Weewan Arsheewa

Woranich Hinthong

Pornpan Pumirat

Nitat Sookrung

Nitaya Indrawattana

Affiliations

Soon will be listed here.

Abstract

Importance: In this study, we aimed to investigate genetic variation and CPE among bla carrying isolates recovered from Prapokklao Hospital, Chanthaburi Province, Thailand, during 2016-2017. A total of 122 carbapenem-resistant Enterobacterales (CRE) were recovered from clinical samples in Prapokklao Hospital. All CRE samples were confirmed by standard biochemical tests and minimum inhibitory concentration (MIC) test strips (E-test). The carbapenemase production was determined using the modified Hodge test (MHT), the modified carbapenem inactivation method (mCIM), and EDTA-CIM (eCIM). Three single mutations (E168Q, S171A, and R214S) were characterized in this study. This mutation might reflect the hydrolysis of the modified β-lactam spectrum, especially carbapenem, by OXA-48-like. Our report provides evidence of the bla point mutation and carbapenemase-producing phenotype of CRE detected in this healthcare setting. Effective control measures and active surveillance of drug resistance in nosocomial pathogens are crucial for controlling diseases associated with difficult-to-treat bacteria.

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