Genetic Features of the Widespread Plasmid Coding for the Carbapenemase OXA-48

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 2011 Nov 16

PMID 22083465

Citations 169

Authors

Laurent Poirel

Remy A Bonnin

Patrice Nordmann

Affiliations

Soon will be listed here.

Abstract

Complete sequencing of plasmid pOXA-48a carrying the bla(OXA-48) gene from a Klebsiella pneumoniae isolate was performed. Its backbone corresponded to that of an IncL/M-type plasmid, in which the bla(OXA-48) gene had been integrated through the acquisition of the Tn1999 composite transposon without any other antibiotic resistance gene. Molecular epidemiology using a collection of international OXA-48 producers revealed the wide diffusion of pOXA-48a or closely related plasmids.

Citing Articles

Mobile genetic elements encoding antibiotic resistance genes and virulence genes in : important pathways for the acquisition of virulence and resistance.

Han B, Feng C, Jiang Y, Ye C, Wei Y, Liu J Front Microbiol. 2025; 16:1529157.

PMID: 40066273 PMC: 11891212. DOI: 10.3389/fmicb.2025.1529157.

Sequencing Analysis of Invasive Carbapenem-Resistant Isolates Secondary to Gastrointestinal Colonization.

Maugeri G, Calvo M, Bongiorno D, Bivona D, Migliorisi G, Privitera G Microorganisms. 2025; 13(1).

PMID: 39858857 PMC: 11767272. DOI: 10.3390/microorganisms13010089.

An eco-evolutionary perspective on antimicrobial resistance in the context of One Health.

Bustamante M, Mei S, Daras I, van Doorn G, Falcao Salles J, de Vos M iScience. 2025; 28(1):111534.

PMID: 39801834 PMC: 11719859. DOI: 10.1016/j.isci.2024.111534.

A plasmid-chromosome crosstalk in multidrug resistant enterobacteria.

Toribio-Celestino L, Calvo-Villamanan A, Herencias C, Alonso-Del Valle A, Sastre-Dominguez J, Quesada S Nat Commun. 2024; 15(1):10859.

PMID: 39738078 PMC: 11686079. DOI: 10.1038/s41467-024-55169-y.

The dissemination of multidrug-resistant and hypervirulent clones across the Kingdom of Saudi Arabia.

Huang J, Alhejaili A, Alkherd U, Milner M, Zhou G, Alzahrani D Emerg Microbes Infect. 2024; 13(1):2427793.

PMID: 39508718 PMC: 11583321. DOI: 10.1080/22221751.2024.2427793.

References

Potron A, Nordmann P, Lafeuille E, Maskari Z, Al Rashdi F, Poirel L . Characterization of OXA-181, a carbapenem-hydrolyzing class D beta-lactamase from Klebsiella pneumoniae. Antimicrob Agents Chemother. 2011; 55(10):4896-9. PMC: 3186949. DOI: 10.1128/AAC.00481-11. View

Ho P, Lo W, Yeung M, Lin C, Chow K, Ang I . Complete sequencing of pNDM-HK encoding NDM-1 carbapenemase from a multidrug-resistant Escherichia coli strain isolated in Hong Kong. PLoS One. 2011; 6(3):e17989. PMC: 3061923. DOI: 10.1371/journal.pone.0017989. View

Poirel L, Ros A, Carricajo A, Berthelot P, Pozzetto B, Bernabeu S . Extremely drug-resistant Citrobacter freundii isolate producing NDM-1 and other carbapenemases identified in a patient returning from India. Antimicrob Agents Chemother. 2010; 55(1):447-8. PMC: 3019622. DOI: 10.1128/AAC.01305-10. View

Kieser T . Factors affecting the isolation of CCC DNA from Streptomyces lividans and Escherichia coli. Plasmid. 1984; 12(1):19-36. DOI: 10.1016/0147-619x(84)90063-5. View

Heidelberg J, Paulsen I, Nelson K, Gaidos E, Nelson W, Read T . Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis. Nat Biotechnol. 2002; 20(11):1118-23. DOI: 10.1038/nbt749. View

Moquet O, Bouchiat C, Kinana A, Seck A, Arouna O, Bercion R . Class D OXA-48 carbapenemase in multidrug-resistant enterobacteria, Senegal. Emerg Infect Dis. 2011; 17(1):143-4. PMC: 3204621. DOI: 10.3201/eid1701.100244. View

Choi K, Kumar A, Schweizer H . A 10-min method for preparation of highly electrocompetent Pseudomonas aeruginosa cells: application for DNA fragment transfer between chromosomes and plasmid transformation. J Microbiol Methods. 2005; 64(3):391-7. DOI: 10.1016/j.mimet.2005.06.001. View

Poirel L, Bonnin R, Nordmann P . Genetic basis of antibiotic resistance in pathogenic Acinetobacter species. IUBMB Life. 2011; 63(12):1061-7. DOI: 10.1002/iub.532. View

Poirel L, Castanheira M, Carrer A, Rodriguez C, Jones R, Smayevsky J . OXA-163, an OXA-48-related class D β-lactamase with extended activity toward expanded-spectrum cephalosporins. Antimicrob Agents Chemother. 2011; 55(6):2546-51. PMC: 3101449. DOI: 10.1128/AAC.00022-11. View

10.

Aubert D, Naas T, Heritier C, Poirel L, Nordmann P . Functional characterization of IS1999, an IS4 family element involved in mobilization and expression of beta-lactam resistance genes. J Bacteriol. 2006; 188(18):6506-14. PMC: 1595497. DOI: 10.1128/JB.00375-06. View

11.

Cuzon G, Ouanich J, Gondret R, Naas T, Nordmann P . Outbreak of OXA-48-positive carbapenem-resistant Klebsiella pneumoniae isolates in France. Antimicrob Agents Chemother. 2011; 55(5):2420-3. PMC: 3088266. DOI: 10.1128/AAC.01452-10. View

12.

Potron A, Poirel L, Nordmann P . Origin of OXA-181, an emerging carbapenem-hydrolyzing oxacillinase, as a chromosomal gene in Shewanella xiamenensis. Antimicrob Agents Chemother. 2011; 55(9):4405-7. PMC: 3165332. DOI: 10.1128/AAC.00681-11. View

13.

Potron A, Poirel L, Nordmann P . Plasmid-mediated transfer of the bla(NDM-1) gene in Gram-negative rods. FEMS Microbiol Lett. 2011; 324(2):111-6. DOI: 10.1111/j.1574-6968.2011.02392.x. View

14.

Carattoli A, Bertini A, Villa L, Falbo V, Hopkins K, Threlfall E . Identification of plasmids by PCR-based replicon typing. J Microbiol Methods. 2005; 63(3):219-28. DOI: 10.1016/j.mimet.2005.03.018. View

15.

Goren M, Chmelnitsky I, Carmeli Y, Navon-Venezia S . Plasmid-encoded OXA-48 carbapenemase in Escherichia coli from Israel. J Antimicrob Chemother. 2010; 66(3):672-3. DOI: 10.1093/jac/dkq467. View

16.

Castanheira M, Deshpande L, Mathai D, Bell J, Jones R, Mendes R . Early dissemination of NDM-1- and OXA-181-producing Enterobacteriaceae in Indian hospitals: report from the SENTRY Antimicrobial Surveillance Program, 2006-2007. Antimicrob Agents Chemother. 2010; 55(3):1274-8. PMC: 3067112. DOI: 10.1128/AAC.01497-10. View

17.

Cuzon G, Naas T, Lesenne A, Benhamou M, Nordmann P . Plasmid-mediated carbapenem-hydrolysing OXA-48 beta-lactamase in Klebsiella pneumoniae from Tunisia. Int J Antimicrob Agents. 2010; 36(1):91-3. DOI: 10.1016/j.ijantimicag.2010.02.014. View

18.

Poirel L, Naas T, Nordmann P . Diversity, epidemiology, and genetics of class D beta-lactamases. Antimicrob Agents Chemother. 2009; 54(1):24-38. PMC: 2798486. DOI: 10.1128/AAC.01512-08. View

19.

Carrer A, Poirel L, Yilmaz M, Akan O, Feriha C, Cuzon G . Spread of OXA-48-encoding plasmid in Turkey and beyond. Antimicrob Agents Chemother. 2010; 54(3):1369-73. PMC: 2825965. DOI: 10.1128/AAC.01312-09. View

20.

Bailey J, Pinyon J, Anantham S, Hall R . Distribution of the blaTEM gene and blaTEM-containing transposons in commensal Escherichia coli. J Antimicrob Chemother. 2011; 66(4):745-51. DOI: 10.1093/jac/dkq529. View