Low Power Laser Irradiation and Human Adipose-derived Stem Cell Treatments Promote Bone Regeneration in Critical-sized Calvarial Defects in Rats

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2018 Apr 6

PMID 29621288

Citations 19

Authors

Yan-Hsiung Wang

Jyun-Yi Wu

Su Chii Kong

Min-Hsuan Chiang

Mei-Ling Ho

Ming-Long Yeh

Chia-Hsin Chen

Affiliations

Soon will be listed here.

Abstract

Both stem cell therapy and physical treatments have been shown to be beneficial in accelerating bone healing. However, the efficacy of combined treatment with stem cells and physical stimuli for large bone defects remains uncertain. The aim of this study was to evaluate the bone regeneration effects of low-power laser irradiation (LPLI) and human adipose-derived stem cell (ADSC) treatments during fracture repair using a comparative rat calvarial defect model. We evaluated the viability of human ADSCs, which were cultured on a porous PLGA scaffold using an MTS assay. The critical-sized calvarial bone defect rats were divided into 4 groups: control group, LPLI group, ADSC group, and ADSC+LPLI group. Bone formation was evaluated using micro-CT. New bone formation areas and osteogenic factor expression levels were then examined by histomorphological analysis and immunohistochemical staining. Our data showed that PLGA had no cytotoxic effect on human ADSCs. Micro-CT analyses revealed that both the LPLI and ADSC groups showed improved calvarial bone defect healing compared to the control group. In addition, the ADSC+LPLI group showed significantly increased bone volume at 16 weeks after surgery. The area of new bone formation ranked as follows: control group < LPLI group < ADSC group < ADSC+LPLI group. There were significant differences between the groups. In addition, both ADSC and ADSC+LPLI groups showed strong signals of vWF expression. ADSC and LPLI treatments improved fracture repair in critical-sized calvarial defects in rats. Importantly, the combined treatment of ADSCs and LPLI further enhances the bone healing process.

Citing Articles

Bone Regeneration of Rat Critical-Sized Calvarial Defects by the Combination of Photobiomodulation and Adipose-Derived Mesenchymal Stem Cells.

Fekrazad S, Farzad-Mohajeri S, Mashhadiabbas F, Daghighi H, Arany P, Fekrazad R J Lasers Med Sci. 2024; 15:e31.

PMID: 39193112 PMC: 11348449. DOI: 10.34172/jlms.2024.31.

Combined Light and Thermal Stimulation of Bone Marrow Stem Cells.

Chailakhyan R, Grosheva A, Vorobieva N, Yusupov V, Sviridov A J Lasers Med Sci. 2024; 15:e8.

PMID: 39050999 PMC: 11267100. DOI: 10.34172/jlms.2024.08.

Biomaterials combined with ADSCs for bone tissue engineering: current advances and applications.

Song Y, Wang N, Shi H, Zhang D, Wang Q, Guo S Regen Biomater. 2023; 10:rbad083.

PMID: 37808955 PMC: 10551240. DOI: 10.1093/rb/rbad083.

Photobiostimulation conjugated with stem cells or their secretome for temporomandibular joint arthritis in a rat model.

El-Qashty R, Elkashty O, Hany E BMC Oral Health. 2023; 23(1):720.

PMID: 37798702 PMC: 10552280. DOI: 10.1186/s12903-023-03466-1.

Nd:YAG-photobiomodulation enhanced ADSCs multilineage differentiation and immunomodulation potentials.

He L, Zheng Y, Liu M, Dong X, Shen L, He Y Lasers Med Sci. 2023; 38(1):190.

PMID: 37608016 PMC: 10444653. DOI: 10.1007/s10103-023-03818-x.

References

Dmitrieva R, Minullina I, Bilibina A, Tarasova O, Anisimov S, Zaritskey A . Bone marrow- and subcutaneous adipose tissue-derived mesenchymal stem cells: differences and similarities. Cell Cycle. 2011; 11(2):377-83. DOI: 10.4161/cc.11.2.18858. View

Burastero G, Scarfi S, Ferraris C, Fresia C, Sessarego N, Fruscione F . The association of human mesenchymal stem cells with BMP-7 improves bone regeneration of critical-size segmental bone defects in athymic rats. Bone. 2010; 47(1):117-26. DOI: 10.1016/j.bone.2010.03.023. View

Wu J, Chen C, Yeh L, Yeh M, Ting C, Wang Y . Low-power laser irradiation promotes the proliferation and osteogenic differentiation of human periodontal ligament cells via cyclic adenosine monophosphate. Int J Oral Sci. 2013; 5(2):85-91. PMC: 3707076. DOI: 10.1038/ijos.2013.38. View

Ninomiya T, Miyamoto Y, Ito T, Yamashita A, Wakita M, Nishisaka T . High-intensity pulsed laser irradiation accelerates bone formation in metaphyseal trabecular bone in rat femur. J Bone Miner Metab. 2003; 21(2):67-73. DOI: 10.1007/s007740300011. View

Behr B, Tang C, Germann G, Longaker M, Quarto N . Locally applied vascular endothelial growth factor A increases the osteogenic healing capacity of human adipose-derived stem cells by promoting osteogenic and endothelial differentiation. Stem Cells. 2011; 29(2):286-96. PMC: 3400547. DOI: 10.1002/stem.581. View

Schindl A, Schindl M, Kerschan K, Knobler R, Schindl L . Diabetic neuropathic foot ulcer: successful treatment by low-intensity laser therapy. Dermatology. 1999; 198(3):314-6. DOI: 10.1159/000018140. View

Reid I . Relationships between fat and bone. Osteoporos Int. 2007; 19(5):595-606. DOI: 10.1007/s00198-007-0492-z. View

El-Maghraby E, El-Rouby D, Saafan A . Assessment of the effect of low-energy diode laser irradiation on gamma irradiated rats' mandibles. Arch Oral Biol. 2012; 58(7):796-805. DOI: 10.1016/j.archoralbio.2012.10.003. View

Colnot C . Cellular and molecular interactions regulating skeletogenesis. J Cell Biochem. 2005; 95(4):688-97. DOI: 10.1002/jcb.20449. View

10.

Paino F, La Noce M, Di Nucci D, Nicoletti G, Salzillo R, De Rosa A . Human adipose stem cell differentiation is highly affected by cancer cells both in vitro and in vivo: implication for autologous fat grafting. Cell Death Dis. 2017; 8(1):e2568. PMC: 5386348. DOI: 10.1038/cddis.2016.308. View

11.

Kim H, Choi K, Kweon O, Kim W . Enhanced wound healing effect of canine adipose-derived mesenchymal stem cells with low-level laser therapy in athymic mice. J Dermatol Sci. 2012; 68(3):149-56. DOI: 10.1016/j.jdermsci.2012.09.013. View

12.

Costa-Almeida R, Granja P, Soares R, Guerreiro S . Cellular strategies to promote vascularisation in tissue engineering applications. Eur Cell Mater. 2014; 28:51-66. DOI: 10.22203/ecm.v028a05. View

13.

Nakao N, Nakayama T, Yahata T, Muguruma Y, Saito S, Miyata Y . Adipose tissue-derived mesenchymal stem cells facilitate hematopoiesis in vitro and in vivo: advantages over bone marrow-derived mesenchymal stem cells. Am J Pathol. 2010; 177(2):547-54. PMC: 2913350. DOI: 10.2353/ajpath.2010.091042. View

14.

De Francesco F, Tirino V, Desiderio V, Ferraro G, DAndrea F, Giuliano M . Human CD34/CD90 ASCs are capable of growing as sphere clusters, producing high levels of VEGF and forming capillaries. PLoS One. 2009; 4(8):e6537. PMC: 2717331. DOI: 10.1371/journal.pone.0006537. View

15.

Fujihara N, Hiraki K, Marques M . Irradiation at 780 nm increases proliferation rate of osteoblasts independently of dexamethasone presence. Lasers Surg Med. 2006; 38(4):332-6. DOI: 10.1002/lsm.20298. View

16.

Liu Y, Chan J, Teoh S . Review of vascularised bone tissue-engineering strategies with a focus on co-culture systems. J Tissue Eng Regen Med. 2012; 9(2):85-105. DOI: 10.1002/term.1617. View

17.

Kazancioglu H, Ezirganli S, Aydin M . Effects of laser and ozone therapies on bone healing in the calvarial defects. J Craniofac Surg. 2013; 24(6):2141-6. DOI: 10.1097/SCS.0b013e3182a244ae. View

18.

Geuze R, Theyse L, Kempen D, Hazewinkel H, Kraak H, Oner F . A differential effect of bone morphogenetic protein-2 and vascular endothelial growth factor release timing on osteogenesis at ectopic and orthotopic sites in a large-animal model. Tissue Eng Part A. 2012; 18(19-20):2052-62. PMC: 3463278. DOI: 10.1089/ten.TEA.2011.0560. View

19.

Zuk P, Zhu M, Mizuno H, Huang J, Futrell J, Katz A . Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng. 2001; 7(2):211-28. DOI: 10.1089/107632701300062859. View

20.

de Oliveira F, Matos A, Matsuda S, Buzalaf M, Bagnato V, Machado M . Low level laser therapy modulates viability, alkaline phosphatase and matrix metalloproteinase-2 activities of osteoblasts. J Photochem Photobiol B. 2017; 169:35-40. DOI: 10.1016/j.jphotobiol.2017.02.020. View