Adipose-derived Stem Cell Sheets Accelerate Bone Healing in Rat Femoral Defects

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2019 Mar 29

PMID 30921414

Citations 21

Authors

Yasuhisa Yoshida

Hidenori Matsubara

Xiang Fang

Katsuhiro Hayashi

Issei Nomura

Shuhei Ugaji

Tomo Hamada

Hiroyuki Tsuchiya

Affiliations

Soon will be listed here.

Abstract

In the present study, we investigated whether both adipose-derived stem cell (ADSC) and osteogenic-induced ADSC sheets could promote bone healing in a rat distal femoral metaphysis bone defect model. A through-hole defect of 1 mm diameter was drilled into each distal femur of 12 week old rats. Forty-five rats were randomly assigned to three groups: (1) control group; (2) ADSC sheet group; or (3) osteogenic-induced ADSC sheet group. We evaluated each group by analysis of computerized tomography scans every week after the surgery, histological analysis, and DiI labeling (a method of membrane staining for post implant cell tracing). Radiological and histological evaluations showed that a part of the hole persisted in the control group at four weeks after surgery, whereas the hole was restored almost completely by new bone formation in both sheet groups. The mean value of bone density (in Houndsfield units) for the bone defect area was significantly higher in both sheet groups than that in the control group (p = 0.05) at four weeks postoperative. A large number of osteocalcin positive osteoblasts were observed at the area of bone defect, especially in the osteogenic-induced ADCS sheet group. DiI labeling in the newly formed bone showed that each sheet had differentiated into bone tissue at four weeks after surgery. The ADSC and the osteogenic-induced ADSC sheets promoted significantly quicker bone healing in the bone defect. Moreover, the osteogenic-induced ADSC sheet may be more advantageous for bone healing than the ADSC sheet because of the higher number of osteocalcin positive osteoblasts via the transplantation.

Citing Articles

Combination of mesenchymal stem cell sheet with poly-caprolactone nanofibrous mat and Gelfoam increased osteogenesis capacity in rat calvarial defect.

Banimohamad-Shotorbani B, Rahbarghazi R, Jarolmasjed S, Mehdipour A, Shafaei H Bioimpacts. 2025; 15:30006.

PMID: 39963571 PMC: 11830138. DOI: 10.34172/bi.30006.

Unlocking the regenerative key: Targeting stem cell factors for bone renewal.

Karima G, Kim H J Tissue Eng. 2024; 15:20417314241287491.

PMID: 39479284 PMC: 11523181. DOI: 10.1177/20417314241287491.

Role of Adipose-Derived Mesenchymal Stem Cells in Bone Regeneration.

Lau C, Park S, Ethiraj L, Singh P, Raj G, Quek J Int J Mol Sci. 2024; 25(12).

PMID: 38928517 PMC: 11204188. DOI: 10.3390/ijms25126805.

The extracts of osteoblast developed from adipose-derived stem cell and its role in osteogenesis.

Tangporncharoen R, Silathapanasakul A, Tragoonlugkana P, Pruksapong C, Tawonsawatruk T, Supokawej A J Orthop Surg Res. 2024; 19(1):255.

PMID: 38650022 PMC: 11034088. DOI: 10.1186/s13018-024-04747-3.

High quality repair of osteochondral defects in rats using the extracellular matrix of antler stem cells.

Wang Y, Chu W, Zhai J, Wang W, He Z, Zhao Q World J Stem Cells. 2024; 16(2):176-190.

PMID: 38455106 PMC: 10915955. DOI: 10.4252/wjsc.v16.i2.176.

References

Ueyama T, Yamamoto Y, Ueda K, Yajima A, Maeda Y, Yamashita Y . Is gastrectomy-induced high turnover of bone with hyperosteoidosis and increase of mineralization a typical osteomalacia?. PLoS One. 2013; 8(6):e65685. PMC: 3679169. DOI: 10.1371/journal.pone.0065685. View

De Long Jr W, Einhorn T, Koval K, McKee M, Smith W, Sanders R . Bone grafts and bone graft substitutes in orthopaedic trauma surgery. A critical analysis. J Bone Joint Surg Am. 2007; 89(3):649-58. DOI: 10.2106/JBJS.F.00465. View

Tsuchiya H, Abdel-Wanis M, Sakurakichi K, Yamashiro T, Tomita K . Osteosarcoma around the knee. Intraepiphyseal excision and biological reconstruction with distraction osteogenesis. J Bone Joint Surg Br. 2002; 84(8):1162-6. DOI: 10.1302/0301-620x.84b8.13330. View

Kundu A, Putnam A . Vitronectin and collagen I differentially regulate osteogenesis in mesenchymal stem cells. Biochem Biophys Res Commun. 2006; 347(1):347-57. DOI: 10.1016/j.bbrc.2006.06.110. View

Spina A, Montella R, Liccardo D, De Rosa A, Laino L, Mitsiadis T . NZ-GMP Approved Serum Improve hDPSC Osteogenic Commitment and Increase Angiogenic Factor Expression. Front Physiol. 2016; 7:354. PMC: 4990559. DOI: 10.3389/fphys.2016.00354. View

Kim D, Monaco E, Maki A, de Lima A, Kong H, Hurley W . Morphologic and transcriptomic comparison of adipose- and bone-marrow-derived porcine stem cells cultured in alginate hydrogels. Cell Tissue Res. 2010; 341(3):359-70. DOI: 10.1007/s00441-010-1015-3. View

Katsuno T, Ozaki T, Saka Y, Furuhashi K, Kim H, Yasuda K . Low serum cultured adipose tissue-derived stromal cells ameliorate acute kidney injury in rats. Cell Transplant. 2012; 22(2):287-97. DOI: 10.3727/096368912X655019. View

Pereira A, Fernandes R, Carvalho Y, Balducci I, Faig-Leite H . Bone healing in drill hole defects in spontaneously hypertensive male and female rats' femurs. A histological and histometric study. Arq Bras Cardiol. 2007; 88(1):104-9. DOI: 10.1590/s0066-782x2007000100017. View

Matthews B, Naot D, Callon K, Musson D, Locklin R, Hulley P . Enhanced osteoblastogenesis in three-dimensional collagen gels. Bonekey Rep. 2014; 3:560. PMC: 4130130. DOI: 10.1038/bonekey.2014.55. View

10.

Wen Y, Jiang B, Cui J, Li G, Yu M, Wang F . Superior osteogenic capacity of different mesenchymal stem cells for bone tissue engineering. Oral Surg Oral Med Oral Pathol Oral Radiol. 2012; 116(5):e324-32. DOI: 10.1016/j.oooo.2012.02.024. View

11.

Zhu Y, Liu T, Song K, Fan X, Ma X, Cui Z . Adipose-derived stem cell: a better stem cell than BMSC. Cell Biochem Funct. 2008; 26(6):664-75. DOI: 10.1002/cbf.1488. View

12.

Fang X, Murakami H, Demura S, Hayashi K, Matsubara H, Kato S . A novel method to apply osteogenic potential of adipose derived stem cells in orthopaedic surgery. PLoS One. 2014; 9(2):e88874. PMC: 3929506. DOI: 10.1371/journal.pone.0088874. View

13.

Omata K, Matsuno T, Asano K, Hashimoto Y, Tabata Y, Satoh T . Enhanced bone regeneration by gelatin-β-tricalcium phosphate composites enabling controlled release of bFGF. J Tissue Eng Regen Med. 2012; 8(8):604-11. DOI: 10.1002/term.1553. View

14.

Suganuma S, Tada K, Hayashi K, Takeuchi A, Sugimoto N, Ikeda K . Uncultured adipose-derived regenerative cells promote peripheral nerve regeneration. J Orthop Sci. 2012; 18(1):145-51. DOI: 10.1007/s00776-012-0306-9. View

15.

Feng W, Lv S, Cui J, Han X, Du J, Sun J . Histochemical examination of adipose derived stem cells combined with β-TCP for bone defects restoration under systemic administration of 1α,25(OH)2D3. Mater Sci Eng C Mater Biol Appl. 2015; 54:133-41. DOI: 10.1016/j.msec.2015.05.037. View

16.

Kingham P, Kalbermatten D, Mahay D, Armstrong S, Wiberg M, Terenghi G . Adipose-derived stem cells differentiate into a Schwann cell phenotype and promote neurite outgrowth in vitro. Exp Neurol. 2007; 207(2):267-74. DOI: 10.1016/j.expneurol.2007.06.029. View

17.

Barba M, Di Taranto G, Lattanzi W . Adipose-derived stem cell therapies for bone regeneration. Expert Opin Biol Ther. 2017; 17(6):677-689. DOI: 10.1080/14712598.2017.1315403. View

18.

Nomura I, Watanabe K, Matsubara H, Hayashi K, Sugimoto N, Tsuchiya H . Uncultured autogenous adipose-derived regenerative cells promote bone formation during distraction osteogenesis in rats. Clin Orthop Relat Res. 2014; 472(12):3798-806. PMC: 4397752. DOI: 10.1007/s11999-014-3608-8. View

19.

Zuk P, Zhu M, Mizuno H, Huang J, Futrell J, Katz A . Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng. 2001; 7(2):211-28. DOI: 10.1089/107632701300062859. View

20.

Dixit M, Raghuvanshi A, Gupta C, Kureel J, Mansoori M, Shukla P . Medicarpin, a Natural Pterocarpan, Heals Cortical Bone Defect by Activation of Notch and Wnt Canonical Signaling Pathways. PLoS One. 2015; 10(12):e0144541. PMC: 4676632. DOI: 10.1371/journal.pone.0144541. View