Inhibition of Strigolactone Receptors by -phenylanthranilic Acid Derivatives: Structural and Functional Insights

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2018 Mar 11

PMID 29523686

Citations 18

Authors

Cyril Hamiaux

Revel S M Drummond

Zhiwei Luo

Hui Wen Lee

Prachi Sharma

Bart J Janssen

Nigel B Perry

William A Denny

Kimberley C Snowden

Affiliations

Soon will be listed here.

Abstract

The strigolactone (SL) family of plant hormones regulates a broad range of physiological processes affecting plant growth and development and also plays essential roles in controlling interactions with parasitic weeds and symbiotic fungi. Recent progress elucidating details of SL biosynthesis, signaling, and transport offers many opportunities for discovering new plant-growth regulators via chemical interference. Here, using high-throughput screening and downstream biochemical assays, we identified -phenylanthranilic acid derivatives as potent inhibitors of the SL receptors from petunia (DAD2), rice (OsD14), and (AtD14). Crystal structures of DAD2 and OsD14 in complex with inhibitors further provided detailed insights into the inhibition mechanism, and modeling of 19 other plant strigolactone receptors suggested that these compounds are active across a large range of plant species. Altogether, these results provide chemical tools for investigating SL signaling and further define a framework for structure-based approaches to design and validate optimized inhibitors of SL receptors for specific plant targets.

Citing Articles

Effect of histidine covalent modification on strigolactone receptor activation and selectivity.

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Rapid analysis of strigolactone receptor activity in a mutant.

White A, Mendez J, Khosla A, Nelson D Plant Direct. 2022; 6(3):e389.

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