The Effects of Aging on Biosynthetic Processes in the Rat Hypothalamic Osmoregulatory Neuroendocrine System

Overview

Journal Neurobiol Aging

Publisher Elsevier

Specialties Geriatrics
Neurology
Physiology

Date 2018 Mar 2

PMID 29494864

Citations 10

Authors

Michael P Greenwood

Mingkwan Greenwood

Elena V Romanova

Andre S Mecawi

Alex Paterson

Olivera Sarenac

Nina Japundzic-Zigon

Jose Antunes-Rodrigues

Julian F R Paton

Jonathan V Sweedler

David Murphy

Affiliations

Soon will be listed here.

Abstract

Elderly people exhibit a diminished capacity to cope with osmotic challenges such as dehydration. We have undertaken a detailed molecular analysis of arginine vasopressin (AVP) biosynthetic processes in the supraoptic nucleus (SON) of the hypothalamus and secretory activity in the posterior pituitary of adult (3 months) and aged (18 months) rats, to provide a comprehensive analysis of age-associated changes to the AVP system. By matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis, we identified differences in pituitary peptides, including AVP, in adult and aged rats under both basal and dehydrated states. In the SON, increased Avp gene transcription, coincided with reduced Avp promoter methylation in aged rats. Based on transcriptome data, we have previously characterized a number of novel dehydration-induced regulatory factors involved in the response of the SON to osmotic cues. We found that some of these increase in expression with age, while dehydration-induced expression of these genes in the SON was attenuated in aged rats. In summary, we show that aging alters the rat AVP system at the genome, transcriptome, and peptidome levels. These alterations however did not affect circulating levels of AVP in basal or dehydrated states.

Citing Articles

Dysregulation of the fluid homeostasis system by aging.

Jang H, Sharma A, Dan U, Wong J, Knight Z, Garrison J bioRxiv. 2024; .

PMID: 39386575 PMC: 11463352. DOI: 10.1101/2024.09.26.615271.

Transcriptome and methylome of the supraoptic nucleus provides insights into the age-dependent loss of neuronal plasticity.

Thompson D, Odufuwa A, Brissette C, Watt J Front Aging Neurosci. 2023; 15:1223273.

PMID: 37711995 PMC: 10498476. DOI: 10.3389/fnagi.2023.1223273.

Ageing restructures the transcriptome of the hypothalamic supraoptic nucleus and alters the response to dehydration.

Elsamad G, Mecawi A, Pauza A, Gillard B, Paterson A, Duque V NPJ Aging. 2023; 9(1):12.

PMID: 37264028 PMC: 10234251. DOI: 10.1038/s41514-023-00108-2.

Peptidomics.

Hellinger R, Sigurdsson A, Wu W, Romanova E, Li L, Sweedler J Nat Rev Methods Primers. 2023; 3.

PMID: 37250919 PMC: 7614574. DOI: 10.1038/s43586-023-00205-2.

Biagetti B, Puig-Domingo M Aging Dis. 2023; 14(3):605-620.

PMID: 37191429 PMC: 10187696. DOI: 10.14336/AD.2022.1109.

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