Identification of Alcohol Stress Tolerance Genes of Sp. PCC 6803 Using Adaptive Laboratory Evolution

Overview

Journal Biotechnol Biofuels

Publisher Biomed Central

Specialty Biotechnology

Date 2017 Dec 23

PMID 29270221

Citations 12

Authors

Takuya Matsusako

Yoshihiro Toya

Katsunori Yoshikawa

Hiroshi Shimizu

Affiliations

Soon will be listed here.

Abstract

Background: sp. PCC 6803 is an attractive organism for the production of alcohols, such as isobutanol and ethanol. However, because stress against the produced alcohol is a major barrier for industrial applications, it is highly desirable to engineer organisms with strong alcohol tolerance.

Results: Isobutanol-tolerant strains of sp. PCC 6803 were obtained by long-term passage culture experiments using medium containing 2 g/L isobutanol. These evolved strains grew on medium containing 5 g/L isobutanol on which the parental strain could not grow. Mutation analysis of the evolved strains revealed that they acquired resistance ability due to combinatorial malfunctions of slr1044 () and slr0369 (), or slr0322 () and . The tolerant strains demonstrated stress resistance against isobutanol as well as a wide variety of alcohols such as ethanol, -butanol, and isopentanol. As a result of introducing an ethanol-producing pathway into the evolved strain, its productivity successfully increased to 142% of the control strain.

Conclusions: Novel mutations were identified that improved the stress tolerance ability of various alcohols in sp. PCC 6803.

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