Mutations in Hik26 and Slr1916 Lead to High-light Stress Tolerance in Synechocystis Sp. PCC6803

Overview

Journal Commun Biol

Specialty Biology

Date 2021 Mar 17

PMID 33727624

Citations 4

Authors

Katsunori Yoshikawa

Kenichi Ogawa

Yoshihiro Toya

Seiji Akimoto

Fumio Matsuda

Hiroshi Shimizu

Affiliations

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Abstract

Increased tolerance to light stress in cyanobacteria is a desirable feature for their applications. Here, we obtained a high light tolerant (Tol) strain of Synechocystis sp. PCC6803 through an adaptive laboratory evolution, in which the cells were repeatedly sub-cultured for 52 days under high light stress conditions (7000 to 9000 μmol m s). Although the growth of the parental strain almost stopped when exposed to 9000 μmol m s, no growth inhibition was observed in the Tol strain. Excitation-energy flow was affected because of photosystem II damage in the parental strain under high light conditions, whereas the damage was alleviated and normal energy flow was maintained in the Tol strain. The transcriptome data indicated an increase in isiA expression in the Tol strain under high light conditions. Whole genome sequence analysis and reverse engineering revealed two mutations in hik26 and slr1916 involved in high light stress tolerance in the Tol strain.

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