Short-term Changes in Intracellular ROS Localisation After the Silver Nanoparticles Exposure Depending on Particle Size

Overview

Journal Toxicol Rep

Date 2017 Oct 1

PMID 28962392

Citations 23

Authors

Akira Onodera

Fumiko Nishiumi

Kisa Kakiguchi

Atsushi Tanaka

Nami Tanabe

Aki Honma

Katsutoshi Yayama

Yasuo Yoshioka

Kumiko Nakahira

Shigenobu Yonemura

Itaru Yanagihara

Yasuo Tsutsumi

Yuichi Kawai

Affiliations

Soon will be listed here.

Abstract

Silver nanoparticles (AgNPs) induce the production of reactive oxygen species (ROS) and apoptosis. These effects are enhanced by smaller particles. Using live-cell imaging, we show that AgNPs induced ROS production rapidly in a size-dependent manner after exposure of cells to 70-nm and 1-nm AgNPs (AgNPs-70, AgNPs-1), but not AgNO. Exposure of cells to 5 μg/mL each of AgNPs-70, AgNPs-1 or AgNO for 1 h decreased the cell viability by approximately 40%, 100% and 20%, respectively. ROS were rapidly induced after 5 and 60 min by AgNPs-1 and AgNPs-70, respectively, whereas AgNO had no detectable effect. ROS production detected using the reporter dichlorodihydrofluorescein was observed in whole cells and mitochondria 5 and 60 min after exposure to AgNPs-1. The present study is the first, to our knowledge, to report the temporal expression and intracellular localisation of ROS induced by AgNPs.

Citing Articles

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