Pharmacological Insights: Mitochondrial ROS Generation by FNC (Azvudine) in Dalton's Lymphoma Cells Revealed by Super Resolution Imaging

Overview

Journal Cell Biochem Biophys

Specialties Biochemistry
Biophysics
Cell Biology

Date 2024 Mar 14

PMID 38483755

Authors

Naveen Kumar

Vikram Delu

Ilya Ulasov

Sanjay Kumar

Rishi Kant Singh

Sandeep Kumar

Alok Shukla

Anand Kumar Patel

Lokesh Yadav

Ruchi Tiwari

Kumari Rachana

Shivashish Priyadarshi Mohanta

Varsha Singh

Anuradha Yadav

Kaushalendra Kaushalendra

Arbind Acharya

Affiliations

Soon will be listed here.

Abstract

Nucleoside analogs are a common form of chemotherapy that disrupts DNA replication and repair, leading to cell cycle arrest and apoptosis. Reactive oxygen species (ROS) production is a significant mechanism through which these drugs exert their anticancer effects. This study investigated a new nucleoside analog called FNC or Azvudine, and its impact on ROS production and cell viability in Dalton's lymphoma (DL) cells. The study found that FNC treatment resulted in a time- and dose-dependent increase in ROS levels in DL cells. After 15 and 30 min of treatment with 2 and 1 mg/ml of FNC, mitochondrial ROS production was observed in DL cells. Furthermore, prolonged exposure to FNC caused structural alterations and DNA damage in DL cells. The results suggest that FNC's ability to impair DL cell viability may be due to its induction of ROS production and indicate a need for further investigation.

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