Identification of Senescent Cell Surface Targetable Protein DPP4

Overview

Journal Genes Dev

Specialty Molecular Biology

Date 2017 Sep 8

PMID 28877934

Citations 128

Authors

Kyoung Mi Kim

Ji Heon Noh

Monica Bodogai

Jennifer L Martindale

Xiaoling Yang

Fred E Indig

Sandip K Basu

Kei Ohnuma

Chikao Morimoto

Peter F Johnson

Arya Biragyn

Kotb Abdelmohsen

Myriam Gorospe

Affiliations

Soon will be listed here.

Abstract

Senescent cell accumulation in aging tissues is linked to age-associated diseases and declining function, prompting efforts to eliminate them. Mass spectrometry analysis revealed that DPP4 (dipeptidyl peptidase 4) was selectively expressed on the surface of senescent, but not proliferating, human diploid fibroblasts. Importantly, the differential presence of DPP4 allowed flow cytometry-mediated isolation of senescent cells using anti-DPP4 antibodies. Moreover, antibody-dependent cell-mediated cytotoxicity (ADCC) assays revealed that the cell surface DPP4 preferentially sensitized senescent, but not dividing, fibroblasts to cytotoxicity by natural killer cells. In sum, the selective expression of DPP4 on the surface of senescent cells enables their preferential elimination.

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