» Articles » PMID: 28787424

Minimum Information About a Single Amplified Genome (MISAG) and a Metagenome-assembled Genome (MIMAG) of Bacteria and Archaea

Abstract

We present two standards developed by the Genomic Standards Consortium (GSC) for reporting bacterial and archaeal genome sequences. Both are extensions of the Minimum Information about Any (x) Sequence (MIxS). The standards are the Minimum Information about a Single Amplified Genome (MISAG) and the Minimum Information about a Metagenome-Assembled Genome (MIMAG), including, but not limited to, assembly quality, and estimates of genome completeness and contamination. These standards can be used in combination with other GSC checklists, including the Minimum Information about a Genome Sequence (MIGS), Minimum Information about a Metagenomic Sequence (MIMS), and Minimum Information about a Marker Gene Sequence (MIMARKS). Community-wide adoption of MISAG and MIMAG will facilitate more robust comparative genomic analyses of bacterial and archaeal diversity.

Citing Articles

Metagenomic insights of microbial functions under conventional and conservation agriculture.

Thakur S, Gonzalez S, Suravajhala P, Jain S, Yadav S, Narayan K World J Microbiol Biotechnol. 2025; 41(3):100.

PMID: 40064739 DOI: 10.1007/s11274-025-04312-y.


Diversity of sulfur cycling halophiles within the Salton Sea, California's largest lake.

Freund L, Hung C, Topacio T, Diamond C, Fresquez A, Lyons T BMC Microbiol. 2025; 25(1):120.

PMID: 40045185 PMC: 11883979. DOI: 10.1186/s12866-025-03839-2.


Multi-omic stock of surface ocean microbiome built by monthly, weekly and daily sampling in Dapeng Bay, China.

Chen Y, Chen S, Tao J, Li M, Wang W, Chen M Sci Data. 2025; 12(1):378.

PMID: 40038315 PMC: 11880531. DOI: 10.1038/s41597-025-04669-7.


Metabolic pathways associated with Firmicutes prevalence in the gut of multiple livestock animals and humans.

Dias B, Lamarca A, Machado D, Kloh V, de Carvalho F, Vasconcelos A Anim Microbiome. 2025; 7(1):20.

PMID: 40033444 PMC: 11874851. DOI: 10.1186/s42523-025-00379-y.


Exploring the taxonomical and functional profiles of marine microorganisms in Submarine Groundwater Discharge vent water from Mabini, Batangas, Philippines through metagenome-assembled genomes.

Veluz J, Mallari L, Gloria P, Siringan M Front Genet. 2025; 16:1522253.

PMID: 40028274 PMC: 11868764. DOI: 10.3389/fgene.2025.1522253.


References
1.
Imelfort M, Parks D, Woodcroft B, Dennis P, Hugenholtz P, Tyson G . GroopM: an automated tool for the recovery of population genomes from related metagenomes. PeerJ. 2014; 2:e603. PMC: 4183954. DOI: 10.7717/peerj.603. View

2.
Blainey P . The future is now: single-cell genomics of bacteria and archaea. FEMS Microbiol Rev. 2013; 37(3):407-27. PMC: 3878092. DOI: 10.1111/1574-6976.12015. View

3.
Darling A, Jospin G, Lowe E, Matsen 4th F, Bik H, Eisen J . PhyloSift: phylogenetic analysis of genomes and metagenomes. PeerJ. 2014; 2:e243. PMC: 3897386. DOI: 10.7717/peerj.243. View

4.
Reddy T, Thomas A, Stamatis D, Bertsch J, Isbandi M, Jansson J . The Genomes OnLine Database (GOLD) v.5: a metadata management system based on a four level (meta)genome project classification. Nucleic Acids Res. 2014; 43(Database issue):D1099-106. PMC: 4384021. DOI: 10.1093/nar/gku950. View

5.
Sekiguchi Y, Ohashi A, Parks D, Yamauchi T, Tyson G, Hugenholtz P . First genomic insights into members of a candidate bacterial phylum responsible for wastewater bulking. PeerJ. 2015; 3:e740. PMC: 4312070. DOI: 10.7717/peerj.740. View