In Vivo Model of Small Intestine

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2017 Apr 1

PMID 28361322

Citations 10

Authors

Maxime M Mahe

Nicole E Brown

Holly M Poling

Michael A Helmrath

Affiliations

Soon will be listed here.

Abstract

The utilization of human pluripotent stem cells (hPSCs) offers new avenues in the generation of organs and opportunities to understand development and diseases. The hPSC-derived human intestinal organoids (HIOs) provide a new tool to gain insights in small intestinal development, physiology, and associated diseases. Herein, we provide a method for orthotropic transplantation of HIOs in immunocompromised mice. This method highlights the specific steps to successful engraftment and provides insight into the study of bioengineered human small intestine.

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