Use of Lectins As Probes for Analyzing Embryonic Induction

Overview

Journal Wilehm Roux Arch Dev Biol

Specialty Anatomy & Histology

Date 2017 Mar 18

PMID 28305357

Citations 6

Authors

Kenzo Takata

Kiyoko Yamazaki Yamamoto

Ruby Ozawa

Affiliations

Soon will be listed here.

Abstract

Lectins were used as probes to investigate the mechanism of embryonic induction. Concanavalin (Con A) and gorse agglutinin out of 7 species of lectins tested were found to have strong neural-inducing effect on the presumptive ectoderm of newt gastrulae. Their effects were abolished by the addition of α-methyl-D-mannoside and α-L-fucose, respectively. Succinyl-Con A had a weak inducing activity in comparison to Con A. Autoradiography ofH-Con A-treated explants revealed that Con A bound to the inner surface, but not to the outer surface of ectoderm and was successively incorporated into cytoplasm.H-Thymidine incorporation was lower in the first half and higher in the second half of the 60 h cultivation period in Con A-treated explants as compared to controls.Con A-Sepharose had a strong inductive effect. This suggests that neural induction is caused through Con A binding to the plasma membrane, but not through incorporation into the cytoplasm of the ectoderm cells.

Citing Articles

Changes in the interior surface of newly mesodermalized ectoderm and its contact activity with competent ectoderm in the newtCynops (Amphibia).

Suzuki A, Miyagawa J, Kuwana T Rouxs Arch Dev Biol. 2017; 199(7):413-422.

PMID: 28305617 DOI: 10.1007/BF01705852.

Neural-inducing activity and glycoprotein synthesis of newly mesodermalized ectoderm in the newtCynops (Amphibia).

Suzuki A, Matsuda J Rouxs Arch Dev Biol. 2017; 199(7):409-412.

PMID: 28305616 DOI: 10.1007/BF01705851.

Isolation of plasma membranes from Xenopus embryos.

Bretzel G, Janeczek J, Born J, John M, Tiedemann H, Tiedemann H Rouxs Arch Dev Biol. 2017; 195(2):117-122.

PMID: 28305446 DOI: 10.1007/BF00456107.

Cell surface changes of the presumptive ectoderm following neural-inducing treatment by concanavalin A.

Yamamoto K, Ozawa R, Takata K, Kitoh J Wilehm Roux Arch Dev Biol. 2017; 190(6):313-319.

PMID: 28305290 DOI: 10.1007/BF00863268.

Electron microscope study of the binding of Con A-gold to superficial and inner ectoderm layers ofXenopus laevis and its relation to the neural-inducing activity of this lectin.

Tacke L, Grunz H Rouxs Arch Dev Biol. 2017; 195(3):158-167.

PMID: 28305250 DOI: 10.1007/BF02439433.

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