Neural-inducing Activity and Glycoprotein Synthesis of Newly Mesodermalized Ectoderm in the NewtCynops (Amphibia)

Overview

Journal Rouxs Arch Dev Biol

Specialty Biology

Date 2017 Mar 18

PMID 28305616

Citations 2

Authors

Akio S Suzuki

Junichi Matsuda

Affiliations

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Abstract

An artificially mesodermalized ectoderm (mE) shows the same properties as the organizer: chordamesoderm formation and neural induction. The neural-inducing activity of the mE was inhibited by treatment with protein synthesis inhibitors (cycloheximide and puromycin) and a specific inhibitor of protein glycosylation (tunicamycin). These antibiotics also inhibited chordamesoderm differentiaton, especiallly that of notochord. Newly synthesized proteins of the mE were compared with those of presumptive ectoderm (pE) using two-dimensional PAGE. There were differences in relative amounts of many protein spots. These results suggest that neural-inducing activity is related to glycoproteins synthesized during the early phase of mesodermalization.

Citing Articles

A useful approach for the screening of active neural-inducing factors.

Suzuki A, Tabata T, Yamamoto Y Rouxs Arch Dev Biol. 2017; 203(6):351-355.

PMID: 28305828 DOI: 10.1007/BF00457806.

Changes in the interior surface of newly mesodermalized ectoderm and its contact activity with competent ectoderm in the newtCynops (Amphibia).

Suzuki A, Miyagawa J, Kuwana T Rouxs Arch Dev Biol. 2017; 199(7):413-422.

PMID: 28305617 DOI: 10.1007/BF01705852.

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