Cell Surface Changes of the Presumptive Ectoderm Following Neural-inducing Treatment by Concanavalin A

Overview

Journal Wilehm Roux Arch Dev Biol

Specialty Anatomy & Histology

Date 2017 Mar 18

PMID 28305290

Citations 3

Authors

Kiyoko Yamazaki Yamamoto

Ruby Ozawa

Kenzo Takata

Junzoh Kitoh

Affiliations

Soon will be listed here.

Abstract

Scanning electron microscopic studies revealed that Concanavalin A (ConA) induces characteristic changes of the cell surface and the cell architecture of the presumptive ectoderm associated with differentiation into neural tissues. In Con A-treated cells, the filopodia with which cells were connected to each other disappeared from the interior (blastocoelic) surface and the cellular adhesivity decreased significantly. Thereafter, the cells underwent from those of the control explants. After cultivation for 60 h, a certain pattern of cell arrangement, which resembled the architecture of neural tissues, was observed among randomly arranged cells in the explants treated with Con A. The morphological changes specifically observed in Con A-treated explants were different from those found in explants treated with succinyl Con A (S-Con A) orDolichos biflorus agglutinin (DBA), which is unable to induce formation of the neural tissues. The molecular organization of the plasma membrane appears to be important in the mechanism of neural induction.

Citing Articles

Changes in the interior surface of newly mesodermalized ectoderm and its contact activity with competent ectoderm in the newtCynops (Amphibia).

Suzuki A, Miyagawa J, Kuwana T Rouxs Arch Dev Biol. 2017; 199(7):413-422.

PMID: 28305617 DOI: 10.1007/BF01705852.

Alteration of cell adhesion system in amphibian ectoderm cells during primary embryonic induction: changes in reaggregation pattern of induced neurectoderm cells and ultrastructural features of the reaggregate.

Suzuki A, Ueno T, Matsusaka T Rouxs Arch Dev Biol. 2017; 195(2):85-91.

PMID: 28305443 DOI: 10.1007/BF00456104.

Electron microscope study of the binding of Con A-gold to superficial and inner ectoderm layers ofXenopus laevis and its relation to the neural-inducing activity of this lectin.

Tacke L, Grunz H Rouxs Arch Dev Biol. 2017; 195(3):158-167.

PMID: 28305250 DOI: 10.1007/BF02439433.

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