Biological Activity of Vegetalizing and Neuralizing Inducing Factors After Binding to BAC-cellulose and CNBr-sepharose

Overview

Journal Wilehm Roux Arch Dev Biol

Specialty Anatomy & Histology

Date 2017 Mar 18

PMID 28305239

Citations 14

Authors

Hildegard Tiedemann

Jochen Born

Affiliations

Soon will be listed here.

Abstract

Covalent binding to bromoacetyl-cellulose inactivates the vegetalizing factor. The bound factor is however still able to form a complex with an inhibitor for the factor. Covalent binding to CNBr-Sepharose likewise inactivates the vegetalizing factor. The neuralizing factor on the other hand is not inactivated when covalently bound to CNBr-Sepharose. When a crude fraction which contains the neuralizng factor as well as the vegetalizing factor is bound to CNBr-Sepharose the vegetalizing activity is greatly decreased whereas the neuralizing activity is not reduced. This suggests that the mechanisms of action of the two factors are quite different. Whereas the vegetalizing factor must be incorporated into the cells, the neuralizing factor interacts with the plasma membrane of competent ectoderm cells.

Citing Articles

Biological activity of the vegetalizing factor: Decrease after coupling to polysaccharide matrix and enzymatic recovery of active factor.

Born J, Grunz H, Tiedemann H, Tiedemann H Wilehm Roux Arch Dev Biol. 2017; 189(1):47-56.

PMID: 28305925 DOI: 10.1007/BF00848566.

A study of cell interactions involved in Pleurodeles waltlii epidermal differentiation.

Shi D, Riou J, Darribere T, Boucaut J Rouxs Arch Dev Biol. 2017; 197(5):247-257.

PMID: 28305784 DOI: 10.1007/BF00380018.

Changes in the interior surface of newly mesodermalized ectoderm and its contact activity with competent ectoderm in the newtCynops (Amphibia).

Suzuki A, Miyagawa J, Kuwana T Rouxs Arch Dev Biol. 2017; 199(7):413-422.

PMID: 28305617 DOI: 10.1007/BF01705852.

Neural-inducing activity and glycoprotein synthesis of newly mesodermalized ectoderm in the newtCynops (Amphibia).

Suzuki A, Matsuda J Rouxs Arch Dev Biol. 2017; 199(7):409-412.

PMID: 28305616 DOI: 10.1007/BF01705851.

Activation of a neuralizing factor in amphibian ectoderm.

John M, Born J, Tiedemann H, Tiedemann H Wilehm Roux Arch Dev Biol. 2017; 193(1):13-18.

PMID: 28305491 DOI: 10.1007/BF00848595.

References

Holtfreter J . . Wilhelm Roux Arch Entwickl Mech Org. 2017; 128(3):584-633. DOI: 10.1007/BF00649865. View

Jagendorf A, PATCHORNIK A, Sela M . USE OF ANTIBODY BOUND TO MODIFIED CELLULOSE AS AN IMMUNOSPECIFIC ADSORBENT OF ANTIGENS. Biochim Biophys Acta. 1963; 78:516-27. DOI: 10.1016/0006-3002(63)90913-2. View

Asashima M . Inducing effects of the presumptive endoderm of successive stages inTriturus alpestris. Wilehm Roux Arch Dev Biol. 2017; 177(4):301-308. DOI: 10.1007/BF00848181. View

Toivonen S, Tarin D, SAXEN L, Tarin P, WARTIOVAARA J . Transfilter studies on neural induction in the newt. Differentiation. 1975; 4(1):1-7. DOI: 10.1111/j.1432-0436.1975.tb01435.x. View

Born J, Geithe H, Tiedemann H, Kocher-Becker U . Isolation of a vegetalizing inducing factor. Hoppe Seylers Z Physiol Chem. 1972; 353(7):1075-84. DOI: 10.1515/bchm2.1972.353.2.1075. View

Born J, Tiedemann H . The mechanism of embryonic induction: isolation of an inhibitor for the vegetalizing factor. Biochim Biophys Acta. 1972; 279(1):174-83. View

Boegman R, Crumpton M . Comparison of immunoadsorbents prepared by coupling sperm-whale myoglobin to a variety of insoluble polymers. Biochem J. 1970; 120(2):373-9. PMC: 1179607. DOI: 10.1042/bj1200373. View

Niebel J, Tiedemann H . Studies on an inhibitor of embryonic differentiation. Characterization by isoelectric focusing and filtration on sephadex. Eur J Biochem. 1973; 32(2):242-6. DOI: 10.1111/j.1432-1033.1973.tb02603.x. View

Becker U, Tiedemann H . [Experiments on the determination of embryonal amphibian tissue by induction substances in solution]. Z Naturforsch B. 1959; 14B:608-9. View

10.

SAXEN L . Transfilter neural induction of amphibian ectoderm. Dev Biol. 1961; 3:140-52. DOI: 10.1016/0012-1606(61)90002-1. View

11.

Cuatrecasas P . Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads. J Biol Chem. 1970; 245(12):3059-65. View

12.

Kocher-Becker U, Tiedemann H . EXOVAGINATION OF NEWT ENDODERM: CELL AFFINITIES ALTERED BY THE MESODERMAL INDUCING FACTOR. Science. 1965; 147(3654):167-9. DOI: 10.1126/science.147.3654.167. View

13.

David G, Chino T, Reisfeld R . Binding of proteins to CNBr-activated sepharose 4B. FEBS Lett. 1974; 43(3):264-6. DOI: 10.1016/0014-5793(74)80657-5. View

14.

VAINIO T, SAXEN L, Toivonen S, Rapola J . The transmission problem in primary embryonic induction. Exp Cell Res. 1962; 27:527-38. DOI: 10.1016/0014-4827(62)90015-0. View

15.

Tiedemann H, Born J, Tiedemann H . Mechanisms of cell differentiation: Affinity of a morphogenetic factor to DNA. Wilhelm Roux Arch Entwickl Mech Org. 2017; 171(2):160-169. DOI: 10.1007/BF00576334. View

16.

Tiedemann H . Factors determining embryonic differentiation. J Cell Physiol. 1968; 72(2):Suppl 1:129+. DOI: 10.1002/jcp.1040720410. View

17.

Tiedemann H, Tiedemann H, Born J, Kocher-Becker U . [Action of sulfhydryl compounds on embryonic inducing factors]. Wilhelm Roux Arch Entwickl Mech Org. 2017; 163(4):316-324. DOI: 10.1007/BF00577018. View