Comparison of Immunoadsorbents Prepared by Coupling Sperm-whale Myoglobin to a Variety of Insoluble Polymers

Overview

Journal Biochem J

Specialty Biochemistry

Date 1970 Nov 1

PMID 5493858

Citations 2

Authors

R J Boegman

M J Crumpton

Affiliations

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Abstract

Immunoadsorbents that were potentially suitable for the fractionation of antibodies to myoglobin were prepared by coupling myoglobin to aminoethylcellulose, bromoacetylcellulose, CM-cellulose, an ethylene-maleic anhydride co-polymer and Sepharose 4B. A comparison of the properties of the preparations indicated that Sepharose-myoglobin was superior, as an immunoadsorbent, to the other conjugates. Elution of adsorbed antibodies with m-propionic acid, pH2.5, gave yields of precipitable antibody higher than those achieved with other dissociating solvents. Propionic acid eluted 60-100% of the antibody adsorbed by Sepharose-myoglobin, and 60-90% of the eluted protein was precipitable antibody.

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