First Report of Tasmanian Sheep Strain (G2) Genotype Isolated from Iranian Goat Using the High Resolution Melting (HRM) Analysis

Overview

Journal Gastroenterol Hepatol Bed Bench

Specialty Gastroenterology

Date 2017 Feb 23

PMID 28224031

Citations 6

Authors

Ahmad Hosseini-Safa

Mohammad Ali Mohag Hegh

Nader Pestechian

Maryam Ganji

Rasoul Mohammadi

Reza Mahmoudi Lamouki

Mohammad Rostami-Nejad

Affiliations

Soon will be listed here.

Abstract

Aim: The present study was aimed to evaluate genotypes isolated from goats using HRM analysis in Isfahan province.

Background: Cystic echincoccosis, so-called hydatidosis, is widespread infection caused by the larval stage of . This is an important zoonotic disease worldwide, especially in the developing countries such as Iran. To date, molecular studies mainly based on the mitochondrial DNA sequences have identified distinct genotypes termed G1-G10 which can differ in some characteristics such as the growth and infectivity to different intermediate hosts or the survival rate in the definitive hosts that are important for the development of control strategies.

Methods: From August to December 2014, 1341 goats were investigated and hydatid cysts were collected from the liver and lungs of 43 infected goats in Isfahan province abattoirs, Isfahan, Iran. Total genomic DNA was extracted from each sample, amplified for the presence of polymorphism of mitochondrial gene coding for cytochrome c oxidase subunit 1 (CO1), using high resolution melting curve (HRM) method.

Results: the results of HRM analysis using the sequence of CO1 gene for 43 isolates from goats showed 31, 2 and 10 isolates were identified as G1, G2, and G3 genotypes, respectively.

Conclusion: G1 is the predominant genotype in the isolated goat samples in Isfahan province, and the presence of G2 strain was reported for the first time in goat in Iran.

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