RNA-Seq Analysis of Chikungunya Virus Infection and Identification of Granzyme A As a Major Promoter of Arthritic Inflammation

Overview

Journal PLoS Pathog

Specialty Microbiology

Date 2017 Feb 17

PMID 28207896

Citations 75

Authors

Jane A C Wilson

Natalie A Prow

Wayne A Schroder

Jonathan J Ellis

Helen E Cumming

Linden J Gearing

Yee Suan Poo

Adam Taylor

Paul J Hertzog

Francesca Di Giallonardo

Linda Hueston

Roger Le Grand

Bing Tang

Thuy T Le

Joy Gardner

Suresh Mahalingam

Pierre Roques

Phillip I Bird

Andreas Suhrbier

Affiliations

Soon will be listed here.

Abstract

Trial Registration: ClinicalTrials.gov NCT00281294.

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References

Ramsauer K, Schwameis M, Firbas C, Mullner M, Putnak R, Thomas S . Immunogenicity, safety, and tolerability of a recombinant measles-virus-based chikungunya vaccine: a randomised, double-blind, placebo-controlled, active-comparator, first-in-man trial. Lancet Infect Dis. 2015; 15(5):519-27. DOI: 10.1016/S1473-3099(15)70043-5. View

Prakash M, Munoz M, Jain R, Tong P, Koskinen A, Regner M . Granzyme B promotes cytotoxic lymphocyte transmigration via basement membrane remodeling. Immunity. 2014; 41(6):960-72. DOI: 10.1016/j.immuni.2014.11.012. View

Kaiserman D, Stewart S, Plasman K, Gevaert K, Van Damme P, Bird P . Identification of Serpinb6b as a species-specific mouse granzyme A inhibitor suggests functional divergence between human and mouse granzyme A. J Biol Chem. 2014; 289(13):9408-17. PMC: 3979379. DOI: 10.1074/jbc.M113.525808. View

Schilte C, Couderc T, Chretien F, Sourisseau M, Gangneux N, Guivel-Benhassine F . Type I IFN controls chikungunya virus via its action on nonhematopoietic cells. J Exp Med. 2010; 207(2):429-42. PMC: 2822618. DOI: 10.1084/jem.20090851. View

ten Berge I, Wever P, Wolbink A, Surachno J, Wertheim P, Spaeny L . Increased systemic levels of soluble granzymes A and B during primary cytomegalovirus infection after renal transplantation. Transplant Proc. 1998; 30(8):3972-4. DOI: 10.1016/s0041-1345(98)01308-6. View

Hallengard D, Lum F, Kummerer B, Lulla A, Lulla V, Garcia-Arriaza J . Prime-boost immunization strategies against Chikungunya virus. J Virol. 2014; 88(22):13333-43. PMC: 4249109. DOI: 10.1128/JVI.01926-14. View

Hansen K, Brenner S, Dudoit S . Biases in Illumina transcriptome sequencing caused by random hexamer priming. Nucleic Acids Res. 2010; 38(12):e131. PMC: 2896536. DOI: 10.1093/nar/gkq224. View

Hildebrand D, Bode K, Riess D, Cerny D, Waldhuber A, Rommler F . Granzyme A produces bioactive IL-1β through a nonapoptotic inflammasome-independent pathway. Cell Rep. 2014; 9(3):910-7. DOI: 10.1016/j.celrep.2014.10.003. View

Sali T, Pryke K, Abraham J, Liu A, Archer I, Broeckel R . Characterization of a Novel Human-Specific STING Agonist that Elicits Antiviral Activity Against Emerging Alphaviruses. PLoS Pathog. 2015; 11(12):e1005324. PMC: 4672893. DOI: 10.1371/journal.ppat.1005324. View

10.

Zaid A, Rulli N, Rolph M, Suhrbier A, Mahalingam S . Disease exacerbation by etanercept in a mouse model of alphaviral arthritis and myositis. Arthritis Rheum. 2011; 63(2):488-91. DOI: 10.1002/art.30112. View

11.

Nakaya H, Gardner J, Poo Y, Major L, Pulendran B, Suhrbier A . Gene profiling of Chikungunya virus arthritis in a mouse model reveals significant overlap with rheumatoid arthritis. Arthritis Rheum. 2012; 64(11):3553-63. PMC: 3836361. DOI: 10.1002/art.34631. View

12.

Ramachandran R, Altier C, Oikonomopoulou K, Hollenberg M . Proteinases, Their Extracellular Targets, and Inflammatory Signaling. Pharmacol Rev. 2016; 68(4):1110-1142. DOI: 10.1124/pr.115.010991. View

13.

Chirathaworn C, Poovorawan Y, Lertmaharit S, Wuttirattanakowit N . Cytokine levels in patients with chikungunya virus infection. Asian Pac J Trop Med. 2013; 6(8):631-4. DOI: 10.1016/S1995-7645(13)60108-X. View

14.

Pinto A, Morahan P, Brinton M, Stewart D, Gavin E . Comparative therapeutic efficacy of recombinant interferons-alpha, -beta, and -gamma against alphatogavirus, bunyavirus, flavivirus, and herpesvirus infections. J Interferon Res. 1990; 10(3):293-8. DOI: 10.1089/jir.1990.10.293. View

15.

Grieves J, Fye J, Harvey S, Grayson J, Hollis T, Perrino F . Exonuclease TREX1 degrades double-stranded DNA to prevent spontaneous lupus-like inflammatory disease. Proc Natl Acad Sci U S A. 2015; 112(16):5117-22. PMC: 4413332. DOI: 10.1073/pnas.1423804112. View

16.

Hoarau J, Gay F, Pelle O, Samri A, Jaffar-Bandjee M, Gasque P . Identical strength of the T cell responses against E2, nsP1 and capsid CHIKV proteins in recovered and chronic patients after the epidemics of 2005-2006 in La Reunion Island. PLoS One. 2013; 8(12):e84695. PMC: 3871564. DOI: 10.1371/journal.pone.0084695. View

17.

Nunes M, Faria N, de Vasconcelos J, Golding N, Kraemer M, de Oliveira L . Emergence and potential for spread of Chikungunya virus in Brazil. BMC Med. 2015; 13:102. PMC: 4433093. DOI: 10.1186/s12916-015-0348-x. View

18.

Meunier E, Broz P . Interferon-inducible GTPases in cell autonomous and innate immunity. Cell Microbiol. 2015; 18(2):168-80. DOI: 10.1111/cmi.12546. View

19.

Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S . Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics. 2012; 28(12):1647-9. PMC: 3371832. DOI: 10.1093/bioinformatics/bts199. View

20.

Lee E, Schultz K, Griffin D . Mice deficient in interferon-gamma or interferon-gamma receptor 1 have distinct inflammatory responses to acute viral encephalomyelitis. PLoS One. 2013; 8(10):e76412. PMC: 3811984. DOI: 10.1371/journal.pone.0076412. View