Model of Host-Pathogen Interaction Dynamics Links In Vivo Optical Imaging and Immune Responses

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 2016 Nov 9

PMID 27821583

Citations 5

Authors

Angelique Ale

Valerie F Crepin

James W Collins

Nicholas Constantinou

Maryam Habibzay

Ann C Babtie

Gad Frankel

Michael P H Stumpf

Affiliations

Soon will be listed here.

Abstract

Tracking disease progression in vivo is essential for the development of treatments against bacterial infection. Optical imaging has become a central tool for in vivo tracking of bacterial population development and therapeutic response. For a precise understanding of in vivo imaging results in terms of disease mechanisms derived from detailed postmortem observations, however, a link between the two is needed. Here, we develop a model that provides that link for the investigation of Citrobacter rodentium infection, a mouse model for enteropathogenic Escherichia coli (EPEC). We connect in vivo disease progression of C57BL/6 mice infected with bioluminescent bacteria, imaged using optical tomography and X-ray computed tomography, to postmortem measurements of colonic immune cell infiltration. We use the model to explore changes to both the host immune response and the bacteria and to evaluate the response to antibiotic treatment. The developed model serves as a novel tool for the identification and development of new therapeutic interventions.

Citing Articles

The type III secretion system effector EspO of enterohaemorrhagic Escherichia coli inhibits apoptosis through an interaction with HAX-1.

Chatterjee S, Lekmeechai S, Constantinou N, Grzybowska E, Kozik Z, Choudhary J Cell Microbiol. 2021; 23(9):e13366.

PMID: 34021690 PMC: 7613270. DOI: 10.1111/cmi.13366.

Intestinal Epithelial Cells and the Microbiome Undergo Swift Reprogramming at the Inception of Colonic Citrobacter rodentium Infection.

Hopkins E, Roumeliotis T, Mullineaux-Sanders C, Choudhary J, Frankel G mBio. 2019; 10(2).

PMID: 30940698 PMC: 6445932. DOI: 10.1128/mBio.00062-19.

The Citrobacter rodentium type III secretion system effector EspO affects mucosal damage repair and antimicrobial responses.

Berger C, Crepin V, Roumeliotis T, Wright J, Serafini N, Pevsner-Fischer M PLoS Pathog. 2018; 14(10):e1007406.

PMID: 30365535 PMC: 6221368. DOI: 10.1371/journal.ppat.1007406.

Host-associated niche metabolism controls enteric infection through fine-tuning the regulation of type 3 secretion.

Connolly J, Slater S, OBoyle N, Goldstone R, Crepin V, Ruano-Gallego D Nat Commun. 2018; 9(1):4187.

PMID: 30305622 PMC: 6180029. DOI: 10.1038/s41467-018-06701-4.

Continual conscious bioluminescent imaging in freely moving somatotransgenic mice.

Karda R, Perocheau D, Suff N, Ng J, Delhove J, Buckley S Sci Rep. 2017; 7(1):6374.

PMID: 28743959 PMC: 5526882. DOI: 10.1038/s41598-017-06696-w.

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