Selenium Single-wavelength Anomalous Diffraction De Novo Phasing Using an X-ray-free Electron Laser

Overview

Journal Nat Commun

Specialty Biology

Date 2016 Nov 5

PMID 27811937

Citations 22

Authors

Mark S Hunter

Chun Hong Yoon

Hasan Demirci

Raymond G Sierra

E Han Dao

Radman Ahmadi

Fulya Aksit

Andrew L Aquila

Halilibrahim Ciftci

Serge Guillet

Matt J Hayes

Thomas J Lane

Meng Liang

Ulf Lundstrom

Jason E Koglin

Paul Mgbam

Yashas Rao

Lindsey Zhang

Soichi Wakatsuki

James M Holton

Sebastien Boutet

Affiliations

Soon will be listed here.

Abstract

Structural information about biological macromolecules near the atomic scale provides important insight into the functions of these molecules. To date, X-ray crystallography has been the predominant method used for macromolecular structure determination. However, challenges exist when solving structures with X-rays, including the phase problem and radiation damage. X-ray-free electron lasers (X-ray FELs) have enabled collection of diffraction information before the onset of radiation damage, yet the majority of structures solved at X-ray FELs have been phased using external information via molecular replacement. De novo phasing at X-ray FELs has proven challenging due in part to per-pulse variations in intensity and wavelength. Here we report the solution of a selenobiotinyl-streptavidin structure using phases obtained by the anomalous diffraction of selenium measured at a single wavelength (Se-SAD) at the Linac Coherent Light Source. Our results demonstrate Se-SAD, routinely employed at synchrotrons for novel structure determination, is now possible at X-ray FELs.

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