Two-colour Serial Femtosecond Crystallography Dataset from Gadoteridol-derivatized Lysozyme for MAD Phasing

Overview

Journal Sci Data

Specialty Science

Date 2017 Dec 13

PMID 29231920

Citations 2

Authors

Alexander Gorel

Koji Motomura

Hironobu Fukuzawa

R Bruce Doak

Marie Luise Grunbein

Mario Hilpert

Ichiro Inoue

Marco Kloos

Gabriela Nass Kovacs

Eriko Nango

Karol Nass

Christopher M Roome

Robert L Shoeman

Rie Tanaka

Kensuke Tono

Lutz Foucar

Yasumasa Joti

Makina Yabashi

So Iwata

Kiyoshi Ueda

Thomas R M Barends

Ilme Schlichting

Affiliations

Soon will be listed here.

Abstract

We provide a detailed description of a gadoteridol-derivatized lysozyme (gadolinium lysozyme) two-colour serial femtosecond crystallography (SFX) dataset for multiple wavelength anomalous dispersion (MAD) structure determination. The data was collected at the Spring-8 Angstrom Compact free-electron LAser (SACLA) facility using a two-colour double-pulse beam to record two diffraction patterns simultaneously in one diffraction image. Gadolinium lysozyme was chosen as a well-established model system that has a very strong anomalous signal. Diffraction patterns from gadolinium lysozyme microcrystals were recorded to a resolution of 1.9 Å in both colours. This dataset is publicly available through the Coherent X-ray Imaging Data Bank (CXIDB) as a resource for algorithm development.

Citing Articles

High-throughput in situ experimental phasing.

Lawrence J, Orlans J, Evans G, Orville A, Foadi J, Aller P Acta Crystallogr D Struct Biol. 2020; 76(Pt 8):790-801.

PMID: 32744261 PMC: 7397491. DOI: 10.1107/S2059798320009109.

SPIND-TC: an indexing method for two-color X-ray diffraction data.

Li X, Li C, Liu H Acta Crystallogr A Found Adv. 2020; 76(Pt 3):369-375.

PMID: 32356787 PMC: 7233013. DOI: 10.1107/S2053273320001916.

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