Deep Sequencing Reveals Persistence of Cell-associated Mumps Vaccine Virus in Chronic Encephalitis

Overview

Journal Acta Neuropathol

Specialty Neurology

Date 2016 Oct 23

PMID 27770235

Citations 21

Authors

Sofia Morfopoulou

Edward T Mee

Sarah M Connaughton

Julianne R Brown

Kimberly Gilmour

W K Kling Chong

W Paul Duprex

Deborah Ferguson

Mike Hubank

Ciaran Hutchinson

Marios Kaliakatsos

Stephen McQuaid

Simon Paine

Vincent Plagnol

Christopher Ruis

Alex Virasami

Hong Zhan

Thomas S Jacques

Silke Schepelmann

Waseem Qasim

Judith Breuer

Affiliations

Soon will be listed here.

Abstract

Routine childhood vaccination against measles, mumps and rubella has virtually abolished virus-related morbidity and mortality. Notwithstanding this, we describe here devastating neurological complications associated with the detection of live-attenuated mumps virus Jeryl Lynn (MuV) in the brain of a child who had undergone successful allogeneic transplantation for severe combined immunodeficiency (SCID). This is the first confirmed report of MuV associated with chronic encephalitis and highlights the need to exclude immunodeficient individuals from immunisation with live-attenuated vaccines. The diagnosis was only possible by deep sequencing of the brain biopsy. Sequence comparison of the vaccine batch to the MuV isolated from brain identified biased hypermutation, particularly in the matrix gene, similar to those found in measles from cases of SSPE. The findings provide unique insights into the pathogenesis of paramyxovirus brain infections.

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