Julianne R Brown
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Explore the profile of Julianne R Brown including associated specialties, affiliations and a list of published articles.
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36
Citations
1079
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Recent Articles
1.
Buddle S, Forrest L, Akinsuyi N, Martin Bernal L, Brooks T, Venturini C, et al.
Genome Med
. 2024 Sep;
16(1):111.
PMID: 39252069
Background: Metagenomics is a powerful approach for the detection of unknown and novel pathogens. Workflows based on Illumina short-read sequencing are becoming established in diagnostic laboratories. However, high sequencing depth...
2.
Lopez-Labrador F, Huber M, Sidorov I, Brown J, Cuypers L, Laenen L, et al.
J Clin Virol
. 2024 Jun;
173:105695.
PMID: 38823290
Metagenomics is gradually being implemented for diagnosing infectious diseases. However, in-depth protocol comparisons for viral detection have been limited to individual sets of experimental workflows and laboratories. In this study,...
3.
Atkinson L, Lee J, Lennon A, Shah D, Storey N, Morfopoulou S, et al.
Heliyon
. 2023 Oct;
9(9):e19854.
PMID: 37809666
Metagenomic next-generation sequencing (mNGS) is an untargeted technique capable of detecting all microbial nucleic acid within a sample. This protocol outlines our wet laboratory method for mNGS of cerebrospinal fluid...
4.
Penner J, Hassell J, Brown J, Mankad K, Storey N, Atkinson L, et al.
J Infect
. 2023 Aug;
87(5):451-458.
PMID: 37557958
No abstract available.
5.
Morfopoulou S, Buddle S, Torres Montaguth O, Atkinson L, Guerra-Assuncao J, Moradi Marjaneh M, et al.
Nature
. 2023 Mar;
617(7961):564-573.
PMID: 36996872
Since its first identification in Scotland, over 1,000 cases of unexplained paediatric hepatitis in children have been reported worldwide, including 278 cases in the UK. Here we report an investigation...
6.
Harris K, Brown J
J Med Microbiol
. 2023 Feb;
71(12).
PMID: 36748452
Molecular techniques are used in the clinical microbiology laboratory to support culture-based diagnosis of infection and are particularly useful for detecting difficult to culture bacteria or following empirical antimicrobial treatment....
7.
Shah D, Brown J, Lee J, Carpenter M, Wall G, Breuer J
J Clin Virol Plus
. 2022 Jun;
2(2):None.
PMID: 35755957
Background: Infections by several DNA viruses can severely impact outcomes in paediatric immunocompromised patients. Current testing, which is generally limited to singleplex qPCR assays, can miss both common and rarer...
8.
Storey N, Brown J, Pereira R, OSullivan D, Huggett J, Williams R, et al.
J Clin Virol Plus
. 2022 Mar;
1(3):100037.
PMID: 35262020
Reverse transcriptase quantitative PCR (RT-qPCR) is the main diagnostic assay used to detect SARS-CoV-2 RNA in respiratory samples. RT-qPCR is performed by specifically targeting the viral genome using complementary oligonucleotides...
9.
de Vries J, Brown J, Fischer N, Sidorov I, Morfopoulou S, Huang J, et al.
J Clin Virol
. 2021 Jul;
141:104908.
PMID: 34273858
Introduction: Metagenomic sequencing is increasingly being used in clinical settings for difficult to diagnose cases. The performance of viral metagenomic protocols relies to a large extent on the bioinformatic analysis....
10.
Brown J, OSullivan D, Shah D, Atkinson L, Pereira R, Whale A, et al.
J Virol Methods
. 2021 Jun;
295:114215.
PMID: 34166701
Background: This study aimed to evaluate the impact of four different reverse transcription quantitative PCR (RT-qPCR) master mixes on the performance of SARS-CoV-2 diagnostic PCRs using three primer/probe assays targeting...