» Articles » PMID: 27660797

Complete Genome Sequence of the Intracellular Bacterial Symbiont TC1 in the Anaerobic Ciliate Trimyema Compressum

Abstract

A free-living ciliate, Trimyema compressum, found in anoxic freshwater environments harbors methanogenic archaea and a bacterial symbiont named TC1 in its cytoplasm. Here, we report the complete genome sequence of the TC1 symbiont, consisting of a 1.59-Mb chromosome and a 35.8-kb plasmid, which was determined using the PacBio RSII sequencer.

Citing Articles

Complete Genome Sequence of " Hydrogeosomobacter endosymbioticus," an Intracellular Bacterial Symbiont of the Anaerobic Ciliate Scuticociliate GW7.

Shiohama Y, Takeshita K, Hirakata Y, Nobu M, Ito M, Shinzato N Microbiol Resour Announc. 2022; 11(2):e0115021.

PMID: 35175121 PMC: 8852324. DOI: 10.1128/mra.01150-21.


Bacterial symbiosis in ciliates (Alveolata, Ciliophora): Roads traveled and those still to be taken.

Fokin S, Serra V J Eukaryot Microbiol. 2022; 69(5):e12886.

PMID: 35006645 PMC: 9539572. DOI: 10.1111/jeu.12886.


Tripartite Symbiosis of an Anaerobic Scuticociliate with Two Hydrogenosome-Associated Endosymbionts, a -Related Alphaproteobacterium and a Methanogenic Archaeon.

Takeshita K, Yamada T, Kawahara Y, Narihiro T, Ito M, Kamagata Y Appl Environ Microbiol. 2019; 85(24).

PMID: 31585988 PMC: 6881808. DOI: 10.1128/AEM.00854-19.


Advantages of genome sequencing by long-read sequencer using SMRT technology in medical area.

Nakano K, Shiroma A, Shimoji M, Tamotsu H, Ashimine N, Ohki S Hum Cell. 2017; 30(3):149-161.

PMID: 28364362 PMC: 5486853. DOI: 10.1007/s13577-017-0168-8.


Symbiosis Studies in Microbial Ecology.

Kamagata Y, Narihiro T Microbes Environ. 2016; 31(3):201-3.

PMID: 27682576 PMC: 5017795. DOI: 10.1264/jsme2.ME3103rh.

References
1.
Siguier P, Perochon J, Lestrade L, Mahillon J, Chandler M . ISfinder: the reference centre for bacterial insertion sequences. Nucleic Acids Res. 2005; 34(Database issue):D32-6. PMC: 1347377. DOI: 10.1093/nar/gkj014. View

2.
McCutcheon J, Moran N . Extreme genome reduction in symbiotic bacteria. Nat Rev Microbiol. 2011; 10(1):13-26. DOI: 10.1038/nrmicro2670. View

3.
Chin C, Alexander D, Marks P, Klammer A, Drake J, Heiner C . Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data. Nat Methods. 2013; 10(6):563-9. DOI: 10.1038/nmeth.2474. View

4.
Shinzato N, Watanabe I, Meng X, Sekiguchi Y, Tamaki H, Matsui T . Phylogenetic analysis and fluorescence in situ hybridization detection of archaeal and bacterial endosymbionts in the anaerobic ciliate trimyema compressum. Microb Ecol. 2007; 54(4):627-36. DOI: 10.1007/s00248-007-9218-1. View

5.
Treangen T, Sommer D, Angly F, Koren S, Pop M . Next generation sequence assembly with AMOS. Curr Protoc Bioinformatics. 2011; Chapter 11:Unit 11.8. PMC: 3072823. DOI: 10.1002/0471250953.bi1108s33. View