Complete Genome Sequence of " Hydrogeosomobacter Endosymbioticus," an Intracellular Bacterial Symbiont of the Anaerobic Ciliate Scuticociliate GW7

Overview

Journal Microbiol Resour Announc

Publisher American Society for Microbiology

Specialty Microbiology

Date 2022 Feb 17

PMID 35175121

Authors

Yasuo Shiohama

Kazutaka Takeshita

Yuga Hirakata

Masaru K Nobu

Michihiro Ito

Naoya Shinzato

Affiliations

Soon will be listed here.

Abstract

The bacterium " Hydrogenosomobacter endosymbioticus" is an intracellular symbiont of anaerobic scuticociliate GW7, which is associated with hydrogenosome together with methanogenic archaea. Here, we report a complete genome sequence of the symbiont consisting of 827 kbp. Knowing this sequence would contribute to the understanding of the metabolic interactions and evolution of the tripartite symbiosis.

Citing Articles

An Evolutionary-Focused Review of the Holosporales (Alphaproteobacteria): Diversity, Host Interactions, and Taxonomic Re-ranking as Holosporineae Subord. Nov.

Castelli M, Petroni G Microb Ecol. 2025; 88(1):15.

PMID: 40085262 DOI: 10.1007/s00248-025-02509-0.

Hepatincolaceae (Alphaproteobacteria) are Distinct From Holosporales and Independently Evolved to Associate With Ecdysozoa.

Castelli M, Gammuto L, Podushkina D, Vecchi M, Altiero T, Clementi E Environ Microbiol. 2025; 27(1):e70028.

PMID: 39797518 PMC: 11724238. DOI: 10.1111/1462-2920.70028.

References

Kang D, Li F, Kirton E, Thomas A, Egan R, An H . MetaBAT 2: an adaptive binning algorithm for robust and efficient genome reconstruction from metagenome assemblies. PeerJ. 2019; 7:e7359. PMC: 6662567. DOI: 10.7717/peerj.7359. View

Wu Y, Simmons B, Singer S . MaxBin 2.0: an automated binning algorithm to recover genomes from multiple metagenomic datasets. Bioinformatics. 2015; 32(4):605-7. DOI: 10.1093/bioinformatics/btv638. View

Bolger A, Lohse M, Usadel B . Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics. 2014; 30(15):2114-20. PMC: 4103590. DOI: 10.1093/bioinformatics/btu170. View

Alneberg J, Bjarnason B, de Bruijn I, Schirmer M, Quick J, Ijaz U . Binning metagenomic contigs by coverage and composition. Nat Methods. 2014; 11(11):1144-6. DOI: 10.1038/nmeth.3103. View

Tanizawa Y, Fujisawa T, Kaminuma E, Nakamura Y, Arita M . DFAST and DAGA: web-based integrated genome annotation tools and resources. Biosci Microbiota Food Health. 2016; 35(4):173-184. PMC: 5107635. DOI: 10.12938/bmfh.16-003. View

Shinzato N, Watanabe I, Meng X, Sekiguchi Y, Tamaki H, Matsui T . Phylogenetic analysis and fluorescence in situ hybridization detection of archaeal and bacterial endosymbionts in the anaerobic ciliate trimyema compressum. Microb Ecol. 2007; 54(4):627-36. DOI: 10.1007/s00248-007-9218-1. View

Takeshita K, Yamada T, Kawahara Y, Narihiro T, Ito M, Kamagata Y . Tripartite Symbiosis of an Anaerobic Scuticociliate with Two Hydrogenosome-Associated Endosymbionts, a -Related Alphaproteobacterium and a Methanogenic Archaeon. Appl Environ Microbiol. 2019; 85(24). PMC: 6881808. DOI: 10.1128/AEM.00854-19. View

Shinzato N, Aoyama H, Saitoh S, Nikoh N, Nakano K, Shimoji M . Complete Genome Sequence of the Intracellular Bacterial Symbiont TC1 in the Anaerobic Ciliate Trimyema compressum. Genome Announc. 2016; 4(5). PMC: 5034148. DOI: 10.1128/genomeA.01032-16. View

Bankevich A, Nurk S, Antipov D, Gurevich A, Dvorkin M, Kulikov A . SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol. 2012; 19(5):455-77. PMC: 3342519. DOI: 10.1089/cmb.2012.0021. View

10.

Tanizawa Y, Fujisawa T, Nakamura Y . DFAST: a flexible prokaryotic genome annotation pipeline for faster genome publication. Bioinformatics. 2017; 34(6):1037-1039. PMC: 5860143. DOI: 10.1093/bioinformatics/btx713. View

11.

Parks D, Imelfort M, Skennerton C, Hugenholtz P, Tyson G . CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. Genome Res. 2015; 25(7):1043-55. PMC: 4484387. DOI: 10.1101/gr.186072.114. View

12.

Chaumeil P, Mussig A, Hugenholtz P, Parks D . GTDB-Tk: a toolkit to classify genomes with the Genome Taxonomy Database. Bioinformatics. 2019; . PMC: 7703759. DOI: 10.1093/bioinformatics/btz848. View