Involvement of MiR-Let7A in Inflammatory Response and Cell Survival/apoptosis Regulated by Resveratrol in THP-1 Macrophage

Overview

Journal Nutr Res Pract

Specialty Nutritional Sciences

Date 2016 Aug 2

PMID 27478543

Citations 19

Authors

Juhyun Song

Mira Jun

Mok-Ryeon Ahn

Oh Yoen Kim

Affiliations

Soon will be listed here.

Abstract

Background/objectives: Resveratrol, a natural polyphenol, has multiple functions in cellular responses including apoptosis, survival, and differentiation. It also participates in the regulation of inflammatory response and oxidative stress. MicroRNA-Let-7A (miR-Let7A), known as a tumor suppressor miRNA, was recently reported to play a crucial role in both inflammation and apoptosis. Therefore, we examined involvement of miR-Let7A in the modulation of inflammation and cell survival/apoptosis regulated by resveratrol.

Materials/methods: mRNA expression of pro-/anti-inflammatory cytokines and sirtuin 1 (SIRT1), and protein expression of apoptosis signal-regulating kinase 1 (ASK1), p-ASK1, and caspase-3 and cleaved caspase-3 were measured, and cell viability and Hoechst/PI staining for apoptosis were observed in Lipopolysaccharide (LPS)-stimulated human THP-1 macrophages with the treatment of resveratrol and/or miR-Let7A overexpression.

Results: Pre-treatment with resveratrol (25-200 µM) resulted in significant recovery of the reduced cell viabilities under LPS-induced inflammatory condition and in markedly increased expression of miR-Let7A in non-stimulated or LPS-stimulated cells. Increased mRNA levels of tumor necrosis factor-α and interleukin (IL)-6 induced by LPS were significantly attenuated, and decreased levels of IL-10 and brain-derived neurotrophic factor were significantly restored by resveratrol and miR-Let7A overexpression, respectively, or in combination. Decreased expression of IL-4 mRNA by LPS stimulation was also significantly increased by miR-Let7A overexpression co-treated with resveratrol. In addition, decreased SIRT1 mRNA levels, and increased p-ASK1 levels and PI-positive cells by LPS stimulation were significantly restored by resveratrol and miR-Let7A overexpression, respectively, or in combination.

Conclusions: miR-Let7A may be involved in the inflammatory response and cell survival/apoptosis modulated by resveratrol in human THP-1 macrophages.

Citing Articles

Sex Differences in Expression of Pro-Inflammatory Markers and miRNAs in a Mouse Model of CVB3 Myocarditis.

Estepa M, Niehues M, Vakhrusheva O, Haritonow N, Ladilov Y, Barcena M Int J Mol Sci. 2024; 25(17).

PMID: 39273613 PMC: 11395254. DOI: 10.3390/ijms25179666.

Decoding the secrets of longevity: unraveling nutraceutical and miRNA-Mediated aging pathways and therapeutic strategies.

Salama R, Eissa N, Doghish A, Abulsoud A, Abdelmaksoud N, Mohammed O Front Aging. 2024; 5:1373741.

PMID: 38605867 PMC: 11007187. DOI: 10.3389/fragi.2024.1373741.

Dietary Epigenetic Modulators: Unravelling the Still-Controversial Benefits of miRNAs in Nutrition and Disease.

Martino E, DOnofrio N, Balestrieri A, Colloca A, Anastasio C, Sardu C Nutrients. 2024; 16(1).

PMID: 38201989 PMC: 10780859. DOI: 10.3390/nu16010160.

Stilbenes, a Versatile Class of Natural Metabolites for Inflammation-An Overview.

Al-Khayri J, Mascarenhas R, Harish H, Gowda Y, Lakshmaiah V, Nagella P Molecules. 2023; 28(9).

PMID: 37175197 PMC: 10180133. DOI: 10.3390/molecules28093786.

An overview of natural products that modulate the expression of non-coding RNAs involved in oxidative stress and inflammation-associated disorders.

Ngum J, Tatang F, Toumeni M, Nguengo S, Simo U, Mezajou C Front Pharmacol. 2023; 14:1144836.

PMID: 37168992 PMC: 10165025. DOI: 10.3389/fphar.2023.1144836.

References

Sawada M, Suzumura A, Hosoya H, Marunouchi T, Nagatsu T . Interleukin-10 inhibits both production of cytokines and expression of cytokine receptors in microglia. J Neurochem. 1999; 72(4):1466-71. DOI: 10.1046/j.1471-4159.1999.721466.x. View

Park E, Jung H, Yang H, Yoo M, Kim C, Kim K . Optimized THP-1 differentiation is required for the detection of responses to weak stimuli. Inflamm Res. 2007; 56(1):45-50. DOI: 10.1007/s00011-007-6115-5. View

Bachis A, Colangelo A, Vicini S, Doe P, De Bernardi M, Brooker G . Interleukin-10 prevents glutamate-mediated cerebellar granule cell death by blocking caspase-3-like activity. J Neurosci. 2001; 21(9):3104-12. PMC: 6762554. View

Bruner-Tran K, Osteen K, Taylor H, Sokalska A, Haines K, Duleba A . Resveratrol inhibits development of experimental endometriosis in vivo and reduces endometrial stromal cell invasiveness in vitro. Biol Reprod. 2010; 84(1):106-12. PMC: 3012565. DOI: 10.1095/biolreprod.110.086744. View

Goldman E, Chen L, Fu H . Activation of apoptosis signal-regulating kinase 1 by reactive oxygen species through dephosphorylation at serine 967 and 14-3-3 dissociation. J Biol Chem. 2003; 279(11):10442-9. DOI: 10.1074/jbc.M311129200. View

Park D, Baek K, Kim J, Lee J, Ryu S, Chin B . Resveratrol inhibits foam cell formation via NADPH oxidase 1- mediated reactive oxygen species and monocyte chemotactic protein-1. Exp Mol Med. 2009; 41(3):171-9. PMC: 2679243. DOI: 10.3858/emm.2009.41.3.020. View

Song J, Cheon S, Jung W, Lee W, Lee J . Resveratrol induces the expression of interleukin-10 and brain-derived neurotrophic factor in BV2 microglia under hypoxia. Int J Mol Sci. 2014; 15(9):15512-29. PMC: 4200860. DOI: 10.3390/ijms150915512. View

Takeda K, Hatai T, Hamazaki T, Nishitoh H, Saitoh M, Ichijo H . Apoptosis signal-regulating kinase 1 (ASK1) induces neuronal differentiation and survival of PC12 cells. J Biol Chem. 2000; 275(13):9805-13. DOI: 10.1074/jbc.275.13.9805. View

Floreani M, Napoli E, Quintieri L, Palatini P . Oral administration of trans-resveratrol to guinea pigs increases cardiac DT-diaphorase and catalase activities, and protects isolated atria from menadione toxicity. Life Sci. 2003; 72(24):2741-50. DOI: 10.1016/s0024-3205(03)00179-6. View

10.

Ghiringhelli F, Rebe C, Hichami A, Delmas D . Immunomodulation and anti-inflammatory roles of polyphenols as anticancer agents. Anticancer Agents Med Chem. 2012; 12(8):852-73. DOI: 10.2174/187152012802650048. View

11.

Lee B, Moon S . Resveratrol inhibits TNF-alpha-induced proliferation and matrix metalloproteinase expression in human vascular smooth muscle cells. J Nutr. 2005; 135(12):2767-73. DOI: 10.1093/jn/135.12.2767. View

12.

Hulsmans M, Holvoet P . MicroRNA-containing microvesicles regulating inflammation in association with atherosclerotic disease. Cardiovasc Res. 2013; 100(1):7-18. DOI: 10.1093/cvr/cvt161. View

13.

Leiro J, Alvarez E, Arranz J, Laguna R, Uriarte E, Orallo F . Effects of cis-resveratrol on inflammatory murine macrophages: antioxidant activity and down-regulation of inflammatory genes. J Leukoc Biol. 2004; 75(6):1156-65. DOI: 10.1189/jlb.1103561. View

14.

Yang Y, Yang L, Liang X, Zhu G . MicroRNA-155 Promotes Atherosclerosis Inflammation via Targeting SOCS1. Cell Physiol Biochem. 2015; 36(4):1371-81. DOI: 10.1159/000430303. View

15.

Tome-Carneiro J, Larrosa M, Yanez-Gascon M, Davalos A, Gil-Zamorano J, Gonzalvez M . One-year supplementation with a grape extract containing resveratrol modulates inflammatory-related microRNAs and cytokines expression in peripheral blood mononuclear cells of type 2 diabetes and hypertensive patients with coronary artery disease. Pharmacol Res. 2013; 72:69-82. DOI: 10.1016/j.phrs.2013.03.011. View

16.

Zhu J, Yong W, Wu X, Yu Y, Lv J, Liu C . Anti-inflammatory effect of resveratrol on TNF-alpha-induced MCP-1 expression in adipocytes. Biochem Biophys Res Commun. 2008; 369(2):471-7. DOI: 10.1016/j.bbrc.2008.02.034. View

17.

Kumar M, Sahu S, Kumar R, Subuddhi A, Maji R, Jana K . MicroRNA let-7 modulates the immune response to Mycobacterium tuberculosis infection via control of A20, an inhibitor of the NF-κB pathway. Cell Host Microbe. 2015; 17(3):345-356. DOI: 10.1016/j.chom.2015.01.007. View

18.

Lavrik I, Golks A, Krammer P . Caspases: pharmacological manipulation of cell death. J Clin Invest. 2005; 115(10):2665-72. PMC: 1236692. DOI: 10.1172/JCI26252. View

19.

Pestka S, Krause C, Sarkar D, Walter M, Shi Y, Fisher P . Interleukin-10 and related cytokines and receptors. Annu Rev Immunol. 2004; 22:929-79. DOI: 10.1146/annurev.immunol.22.012703.104622. View

20.

Stein S, Schafer N, Breitenstein A, Besler C, Winnik S, Lohmann C . SIRT1 reduces endothelial activation without affecting vascular function in ApoE-/- mice. Aging (Albany NY). 2010; 2(6):353-60. PMC: 2919255. DOI: 10.18632/aging.100162. View