Oxidation and Interaction of DJ-1 with 20S Proteasome in the Erythrocytes of Early Stage Parkinson's Disease Patients

Overview

Journal Sci Rep

Specialty Science

Date 2016 Jul 30

PMID 27470541

Citations 17

Authors

Yoshiro Saito

Yoko Akazawa-Ogawa

Akihiro Matsumura

Kazumasa Saigoh

Sayoko Itoh

Kenta Sutou

Mayuka Kobayashi

Yuichiro Mita

Mototada Shichiri

Shin Hisahara

Yasuo Hara

Harutoshi Fujimura

Hiroyuki Takamatsu

Yoshihisa Hagihara

Yasukazu Yoshida

Takao Hamakubo

Susumu Kusunoki

Shun Shimohama

Noriko Noguchi

Affiliations

Soon will be listed here.

Abstract

Parkinson's disease (PD) is a progressive, age-related, neurodegenerative disorder, and oxidative stress is an important mediator in its pathogenesis. DJ-1, the product of the causative gene of a familial form of PD, plays a significant role in anti-oxidative defence to protect cells from oxidative stress. DJ-1 undergoes preferential oxidation at the cysteine residue at position 106 (Cys-106) under oxidative stress. Here, using specific antibodies against Cys-106-oxidized DJ-1 (oxDJ-1), it was found that the levels of oxDJ-1 in the erythrocytes of unmedicated PD patients (n = 88) were higher than in those of medicated PD patients (n = 62) and healthy control subjects (n = 33). Elevated oxDJ-1 levels were also observed in a non-human primate PD model. Biochemical analysis of oxDJ-1 in erythrocyte lysates showed that oxDJ-1 formed dimer and polymer forms, and that the latter interacts with 20S proteasome. These results clearly indicate a biochemical alteration in the blood of PD patients, which could be utilized as an early diagnosis marker for PD.

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