Homoeologue Expression Insights into the Basis of Growth Heterosis at the Intersection of Ploidy and Hybridity in Cyprinidae

Overview

Journal Sci Rep

Specialty Science

Date 2016 Jun 7

PMID 27265401

Citations 13

Authors

Li Ren

Wuhui Li

Min Tao

Qinbo Qin

Jing Luo

Jing Chai

Chenchen Tang

Jun Xiao

Xiaojun Tang

Guoliang Lin

Chun Zhang

Wei Duan

Yunhan Hong

Shaojun Liu

Affiliations

Soon will be listed here.

Abstract

Hybridization and polyploidization are considered important driving forces that form new epigenetic regulations. To study the changing patterns of expression accompanying hybridization and polyploidization, we used RNA-seq and qRT-PCR to investigate global expression and homoeologue expression in diploid and tetraploid hybrids of Carassius auratus red var. (♀) (R) and Cyprinus carpio (♂) (C). By comparing the relative expression levels between the hybrids and their parents, we defined the expression level dominance (ELD) and homoeologue expression bias (HEB) in liver tissue. The results showed that polyploidization contributed to the conversion of homoeologue ELD. In addition, hybridization had more effect on the change in HEB than polyploidization, while polyploidization had more effect on the change of global gene expression than hybridization. Meanwhile, similar expression patterns were found in growth-related genes. The results suggested that hybridization and polyploidization result in differential degrees of maternal HEB in three tissues (liver, muscle and ovary) tested. The results of this study will increase our understanding of the underlying regulation mechanism of rapid growth in diploid hybrids and allotetraploids. The differential degrees of global expression and homoeologue expression contribute to growth heterosis in newly formed hybrids, ensuring the on-going success of allotetraploid speciation.

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