Optimal Reference Genes for Gene Expression Analysis in Polyploid of Cyprinus Carpio and Carassius Auratus

Overview

Journal BMC Genet

Publisher Biomed Central

Specialties Biotechnology
Molecular Biology

Date 2020 Sep 18

PMID 32943013

Citations 2

Authors

Wenbin Liu

Xiudan Yuan

Shuli Yuan

Liuye Dai

Shenghua Dong

Jinhui Liu

Liangyue Peng

Minmeng Wang

Yi Tang

Yamei Xiao

Affiliations

Soon will be listed here.

Abstract

Background: Reference genes are usually stably expressed in various cells and tissues. However, it was reported that the expression of some reference genes may be distinct in different species. In this study, we intend to answer whether the expression of reported traditional reference genes changes or not in the polyploid fish RESULTS: By retrieving the mRNA sequencing data of three different ploidy fish from the NCBI SRA database, we selected 12 candidate reference genes, and examined their expression levels in the 10 tissues and in the four cell lines of three different ploidy fish by real-time PCR. Then, the expression profiles of these 12 candidate reference genes were systematically evaluated by using the software platforms: BestKeeper, NormFinder and geNorm.

Conclusion: The 28S ribosomal protein S5 gene (RPS5) and the ribosomal protein S18 gene (RPS18) are the most suitable reference genes for the polyploid of Cyprinus carpio and Carassius auratus, demonstrated by both of the tissues and the cultured cells.

Citing Articles

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