The Modifier of Transcription 1 (Mot1) ATPase and Spt16 Histone Chaperone Co-regulate Transcription Through Preinitiation Complex Assembly and Nucleosome Organization

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2016 May 27

PMID 27226635

Citations 24

Authors

Jason D True

Joseph J Muldoon

Melissa N Carver

Kunal Poorey

Savera J Shetty

Stefan Bekiranov

David T Auble

Affiliations

Soon will be listed here.

Abstract

Modifier of transcription 1 (Mot1) is a conserved and essential Swi2/Snf2 ATPase that can remove TATA-binding protein (TBP) from DNA using ATP hydrolysis and in so doing exerts global effects on transcription. Spt16 is also essential and functions globally in transcriptional regulation as a component of the facilitates chromatin transcription (FACT) histone chaperone complex. Here we demonstrate that Mot1 and Spt16 regulate a largely overlapping set of genes in Saccharomyces cerevisiae. As expected, Mot1 was found to control TBP levels at co-regulated promoters. In contrast, Spt16 did not affect TBP recruitment. On a global scale, Spt16 was required for Mot1 promoter localization, and Mot1 also affected Spt16 localization to genes. Interestingly, we found that Mot1 has an unanticipated role in establishing or maintaining the occupancy and positioning of nucleosomes at the 5' ends of genes. Spt16 has a broad role in regulating chromatin organization in gene bodies, including those nucleosomes affected by Mot1. These results suggest that the large scale overlap in Mot1 and Spt16 function arises from a combination of both their unique and shared functions in transcription complex assembly and chromatin structure regulation.

Citing Articles

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Transcriptional regulation of FACT involves Coordination of chromatin accessibility and CTCF binding.

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Nucleosome retention by histone chaperones and remodelers occludes pervasive DNA-protein binding.

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The histone chaperone FACT: a guardian of chromatin structure integrity.

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