Drosophila FACT Contributes to Hox Gene Expression Through Physical and Functional Interactions with GAGA Factor

Overview

Journal Genes Dev

Specialty Molecular Biology

Date 2003 Jun 20

PMID 12815073

Citations 58

Authors

Tsukasa Shimojima

Masahiro Okada

Takahiro Nakayama

Hitoshi Ueda

Katsuya Okawa

Akihiro Iwamatsu

Hiroshi Handa

Susumu Hirose

Affiliations

Soon will be listed here.

Abstract

Chromatin structure plays a critical role in the regulation of transcription. Drosophila GAGA factor directs chromatin remodeling to its binding sites. We show here that Drosophila FACT (facilitates chromatin transcription), a heterodimer of dSPT16 and dSSRP1, is associated with GAGA factor through its dSSRP1 subunit, binds to a nucleosome, and facilitates GAGA factor-directed chromatin remodeling. Moreover, genetic interactions between Trithorax-like encoding GAGA factor and spt16 implicate the GAGA factor-FACT complex in expression of Hox genes Ultrabithorax, Sex combs reduced, and Abdominal-B. Chromatin immunoprecipitation experiments indicated the presence of the GAGA factor-FACT complex in the regulatory regions of Ultrabithorax and Abdominal-B. These data illustrate a crucial role of FACT in the modulation of chromatin structure for the regulation of gene expression.

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