Kinesin-8 Effects on Mitotic Microtubule Dynamics Contribute to Spindle Function in Fission Yeast

Overview

Journal Mol Biol Cell

Specialties Cell Biology
Molecular Biology

Date 2016 May 6

PMID 27146110

Citations 18

Authors

Zachary R Gergely

Ammon Crapo

Loren E Hough

J Richard McIntosh

Meredith D Betterton

Affiliations

Soon will be listed here.

Abstract

Kinesin-8 motor proteins destabilize microtubules. Their absence during cell division is associated with disorganized mitotic chromosome movements and chromosome loss. Despite recent work studying effects of kinesin-8s on microtubule dynamics, it remains unclear whether the kinesin-8 mitotic phenotypes are consequences of their effect on microtubule dynamics, their well-established motor activity, or additional, unknown functions. To better understand the role of kinesin-8 proteins in mitosis, we studied the effects of deletion of the fission yeast kinesin-8 proteins Klp5 and Klp6 on chromosome movements and spindle length dynamics. Aberrant microtubule-driven kinetochore pushing movements and tripolar mitotic spindles occurred in cells lacking Klp5 but not Klp6. Kinesin-8-deletion strains showed large fluctuations in metaphase spindle length, suggesting a disruption of spindle length stabilization. Comparison of our results from light microscopy with a mathematical model suggests that kinesin-8-induced effects on microtubule dynamics, kinetochore attachment stability, and sliding force in the spindle can explain the aberrant chromosome movements and spindle length fluctuations seen.

Citing Articles

Quantifying Yeast Microtubules and Spindles Using the Toolkit for Automated Microtubule Tracking (TAMiT).

Ansari S, Gergely Z, Flynn P, Li G, Moore J, Betterton M Biomolecules. 2023; 13(6).

PMID: 37371519 PMC: 10296093. DOI: 10.3390/biom13060939.

The kinesin-5 protein Cut7 moves bidirectionally on fission yeast spindles with activity that increases in anaphase.

Gergely Z, Ansari S, Jones M, Zhou B, Cash C, McIntosh R J Cell Sci. 2023; 136(5).

PMID: 36655493 PMC: 10112985. DOI: 10.1242/jcs.260474.

A force balance model for a cell size-dependent meiotic nuclear oscillation in fission yeast.

Fujita I, Kimura A, Yamashita A EMBO Rep. 2023; 24(3):e55770.

PMID: 36622644 PMC: 9986818. DOI: 10.15252/embr.202255770.

Length-dependent poleward flux of sister kinetochore fibers promotes chromosome alignment.

Risteski P, Bozan D, Jagric M, Bosilj A, Pavin N, Tolic I Cell Rep. 2022; 40(5):111169.

PMID: 35926461 PMC: 9364240. DOI: 10.1016/j.celrep.2022.111169.

Modeling spatiotemporally varying protein-protein interactions in CyLaKS, the Cytoskeleton Lattice-based Kinetic Simulator.

Fiorenza S, Steckhahn D, Betterton M Eur Phys J E Soft Matter. 2021; 44(8):105.

PMID: 34406510 PMC: 10202044. DOI: 10.1140/epje/s10189-021-00097-8.

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